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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 1 - 12 (12page)
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초록· 키워드
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Objectives: The aim of this in vitro study was to evaluate the biocompatibility of newly proposed root-end filling materials, Biodentine, Micro-Mega mineral trioxide aggregate (MM-MTA), polymethylmethacrylate (PMMA) bone cement, and Smart Dentin Replacement (SDR), in comparison with contemporary root-end filling materials, intermediate restorative material (IRM), Dyract compomer, ProRoot MTA (PMTA), and Vitrebond, using human periodontal ligament (hPDL) fibroblasts. Materials and Methods: Ten discs from each material were fabricated in sterile Teflon molds and 24-hour eluates were obtained from each root-end filling material in cell culture media after 1- or 3-day setting. hPDL fibroblasts were plated at a density of 5 × 103 /well, and were incubated for 24 hours with 1:1, 1:2, 1:4, and 1:8 dilutions of eluates. Cell viability was evaluated by XTT assay. Data was statistically analysed. Apoptotic/necrotic activity of PDL cells exposed to material eluates was established by flow cytometry. Results: The Vitrebond and IRM were significantly more cytotoxic than the other root-end filling materials (p < 0.05). Those cells exposed to the Biodentine and Dyract compomer eluates showed the highest survival rates (p < 0.05), while the PMTA, MM-MTA, SDR, and PMMA groups exhibited similar cell viabilities. Three-day samples were more cytotoxic than 1-day samples (p < 0.05). Eluates from the cements at 1:1 dilution were significantly more cytotoxic (p < 0.05). Vitrebond induced cell necrosis as indicated by flow cytometry. Conclusions: This in vitro study demonstrated that Biodentine and Compomer were more biocompatible than the other root-end filling materials. Vitrebond eluate caused necrotic cell death.
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참고문헌 (36)
참고문헌 신청
Kim S / 2006 / Modern endodontic surgery concepts and practice: a review / J Endod 32:601~623
Chong BS / 2003 / A prospective clinical study of Mineral Trioxide Aggregate and IRM when used as root-end filling materials in endodontic surgery / Int Endod J 36:520~526
Ma J / 2011 / Biocompatibility of two novel root repair materials / J Endod 37:793~798
Chong BS / 1997 / Short-term tissue response to potential root-end filling materials in infected root canals / Int Endod J 30:240~249
Mazumdar D / 2005 / An investigation into the irritant properties of some retrograde filling materials-an in vivo study / J Conserv Dent 8:4~13
Chong BS / 2003 / A prospective clinical study of Mineral Trioxide Aggregate and IRM when used as root-end filling materials in endodontic surgery / Int Endod J 36:520~526
Ma J / 2011 / Biocompatibility of two novel root repair materials / J Endod 37:793~798
Chong BS / 1997 / Short-term tissue response to potential root-end filling materials in infected root canals / Int Endod J 30:240~249
Mazumdar D / 2005 / An investigation into the irritant properties of some retrograde filling materials-an in vivo study / J Conserv Dent 8:4~13
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