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논문 기본 정보

자료유형
학술저널
저자정보
Yoon Ji-Hye (Department of Conservative Dentistry School of Dentistry Dental Science Research Institute Chonnam) Choi Sung-Hyeon (Department of Conservative Dentistry School of Dentistry Dental Science Research Institute Chonnam) Koh Jeong-Tae (Department of Pharmacology and Dental Therapeutics School of Dentistry Dental Science Research Inst) Lee Bin-Na (Department of Conservative Dentistry School of Dentistry Dental Science Research Institute Chonnam) Chang Hoon-Sang (Department of Conservative Dentistry School of Dentistry Dental Science Research Institute Chonnam) Hwang In-Nam (Department of Conservative Dentistry School of Dentistry Dental Science Research Institute Chonnam) Oh Won-Mann (Department of Conservative Dentistry School of Dentistry Dental Science Research Institute Chonnam) Hwang Yun-Chan (Department of Conservative Dentistry School of Dentistry Dental Science Research Institute Chonnam)
저널정보
대한치과보존학회 Restorative Dentistry and Endodontics Restorative Dentistry and Endodontics 제46권 제2호
발행연도
2021.1
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1 - 9 (9page)

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Objectives In recent in vitro study, it was reported that osteostatin (OST) has an odontogenic effect and synergistic effect with mineral trioxide aggregate (MTA) in human dental pulp cells. Therefore, the aim of this study was to evaluate whether OST has a synergistic effect with MTA on hard tissue formation in vivo. Materials and Methods Thirty-two maxillary molars of Spraque-Dawley rats were used in this study. An occlusal cavity was prepared and the exposed pulps were randomly divided into 3 groups: group 1 (control; ProRoot MTA), group 2 (OST 100 μM + ProRoot MTA), group 3 (OST 10 mM + ProRoot MTA). Exposed pulps were capped with each material and cavities were restored with resin modified glass ionomer. The animals were sacrificed after 4 weeks. All harvested teeth were scanned with micro-computed tomography (CT). The samples were prepared and hard tissue formation was evaluated histologically. For immunohistochemical analysis, the specimens were sectioned and incubated with primary antibodies against dentin sialoprotein (DSP). Results In the micro-CT analysis, it is revealed that OST with ProRoot MTA groups showed more mineralized bridge than the control (p < 0.05). In the H&E staining, it is showed that more quantity of the mineralized dentin bridge was formed in the OST with ProRoot MTA group compared to the control (p < 0.05). In all groups, DSP was expressed in newly formed reparative dentin area. Conclusions OST can be a supplementary pulp capping material when used with MTA to make synergistic effect in hard tissue formation.
#Direct pulp capping #Mineralization #MTA #Osteostatin

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참고문헌 (30)

참고문헌 신청
An S / 2012 / Calcium ions promote osteogenic differentiation and mineralization of human dental pulp cells: implications for pulp capping materials / J Mater Sci Mater Med 23:789~795 google schola Hilton TJ / 2009 / Keys to clinical success with pulp capping: a review of the literature / Oper Dent 34:615~625 google schola Scarano A / 2003 / Direct capping with four different materials in humans: histological analysis of odontoblast activity / J Endod 29:729~734 google schola Hörsted-Bindslev P / 2003 / Direct capping of human pulps with a dentin bonding system or with calcium hydroxide cement / Oral Surg Oral Med Oral Pathol Oral Radiol Endod 96:591~600 google schola Casella G / 2006 / The use of mineral trioxide aggregate in endodontics / Minerva Stomatol 55:123~143 google schola

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