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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 353 - 357 (5page)
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초록· 키워드
오류제보하기
The effects of storage temperatures, repeated freeze-thaw cycles, or delays in separating plasma or serum from blood samples are largely unknown for heat shock protein 27 (HSP27). We evaluated (1) the imprecision of the HSP27 assay used in this study; (2) the in vitro stability of HSP27 in blood samples stored at 4°C for up to 6 hr with immediate and delayed serum/plasma separation from cells; and (3) the in vitro stability of HSP27 in blood samples stored at -80°C after repeated freeze-thaw cycles. The ELISA to detect HSP27 in this study showed a within-run CV of <9% and a total CV of <15%. After 4-6 hr of storage at 4°C, HSP27 concentrations remained stable when using serum tubes irrespective of sample handling, but HSP27 concentrations decreased by 25-45% when using EDTA plasma tubes. Compared with baseline HSP27, one freeze-thaw cycle had no effect on serum concentrations. However, plasma concentrations increased by 3.1-fold after one freeze-thaw cycle and by 7.3-fold after five freeze-thaw cycles. In conclusion, serum is an appropriate biological sample type for use in epidemiological and large-scale clinical studies.
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등록된 정보가 없습니다.
참고문헌 (15)
참고문헌 신청
Mymrikov EV / 2011 / Large potentials of small heat shock proteins / Physiol Rev 91:1123~1159
Lianos GD / 2015 / The role of heat shock proteins in cancer / Cancer Lett 360:114~118
Ghayour-Mobarhan M / 2012 / The potential role of heat shock protein 27 in cardiovascular disease / Clin Chim Acta 413:15~24
Park HK / 2006 / Expression of heat shock protein 27 in human atherosclerotic plaques and increased plasma level of heat shock protein 27 in patients with acute coronary syndrome / Circulation 114:886~893
Périard JD / 2012 / Plasma Hsp72 (HSPA1A) and Hsp27 (HSPB1) expression under heat stress: influence of exercise intensity / Cell Stress Chaperones 17:375~383
Lianos GD / 2015 / The role of heat shock proteins in cancer / Cancer Lett 360:114~118
Ghayour-Mobarhan M / 2012 / The potential role of heat shock protein 27 in cardiovascular disease / Clin Chim Acta 413:15~24
Park HK / 2006 / Expression of heat shock protein 27 in human atherosclerotic plaques and increased plasma level of heat shock protein 27 in patients with acute coronary syndrome / Circulation 114:886~893
Périard JD / 2012 / Plasma Hsp72 (HSPA1A) and Hsp27 (HSPB1) expression under heat stress: influence of exercise intensity / Cell Stress Chaperones 17:375~383
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