지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 450 - 456 (7page)
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초록· 키워드
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Background: During the 2015 outbreak of Middle East Respiratory Syndrome coronavirus (MERS-CoV), six different commercial MERS-CoV RNA detection kits based on real-time reverse-transcription polymerase chain reaction (rRT-PCR) were available in Korea. We performed analytical and clinical validations of these kits.
Methods: PowerChek (Kogene Biotech, Korea), DiaPlexQ (SolGent, Korea), Anyplex (Seegene, Korea), AccuPower (Bioneer, Korea), LightMix (Roche Molecular Diagnostics, Switzerland), and UltraFast kits (Nanobiosys, Korea) were evaluated. Limits of detection (LOD) with 95% probability values were estimated by testing 16 replicates of upstream of the envelope gene (upE) and open reading frame 1a (ORF1a) RNA transcripts. Specificity was estimated by using 28 nasopharyngeal swabs that were positive for other respiratory viruses. Clinical sensitivity was evaluated by using 18 lower respiratory specimens. The sensitivity test panel and the high inhibition panel were composed of nine specimens each, including eight and six specimens that were positive for MERS-CoV, respectively.
Results: The LODs for upE ranged from 21.88 to 263.03 copies/reaction, and those for ORF1a ranged from 6.92 to 128.82 copies/reaction. No cross-reactivity with other respiratory viruses was found. All six kits correctly identified 8 of 8 (100%) positive clinical specimens. Based on results from the high inhibition panel, PowerChek and AccuPower were the least sensitive to the presence of PCR inhibition.
Conclusions: The overall sensitivity and specificity of all six assay systems were sufficient for diagnosing MERS-CoV infection. However, the analytical sensitivity and detection ability in specimens with PCR inhibition could be improved with the use of appropriate internal controls.
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참고문헌 (14)
참고문헌 신청
Cowling BJ / 2015 / Preliminary epidemiological assessment of MERS-CoV outbreak in South Korea / Euro Surveill 20:7~13
Park HY / 2015 / Epidemiological investigation of MERS-CoV spread in a single hospital in South Korea / Euro Surveill 20:1~6
WHO / Laboratory testing for Middle East Respiratory Syndrome Coronavirus
Center for Disease Control and Prevention / Laboratory testing for Middle East Respiratory Syndrome Coronavirus (MERS-CoV)
Lu X / 2014 / Real-time reverse transcription-PCR assay panel for Middle East respiratory syndrome coronavirus / J Clin Microbiol 52:67~75
Park HY / 2015 / Epidemiological investigation of MERS-CoV spread in a single hospital in South Korea / Euro Surveill 20:1~6
WHO / Laboratory testing for Middle East Respiratory Syndrome Coronavirus
Center for Disease Control and Prevention / Laboratory testing for Middle East Respiratory Syndrome Coronavirus (MERS-CoV)
Lu X / 2014 / Real-time reverse transcription-PCR assay panel for Middle East respiratory syndrome coronavirus / J Clin Microbiol 52:67~75
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