개인구독
소속 기관이 없으신 경우, 개인 정기구독을 하시면 저렴하게
논문을 무제한 열람 이용할 수 있어요.
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 494 - 498 (5page)
이용수
초록· 키워드
오류제보하기
Background: Molecular detection of Middle East respiratory syndrome coronavirus (MERS-CoV) using real-time reverse transcription (rRT)-PCR assays is the method of choice for diagnosis of MERS. We evaluated the performance of the PowerChek MERS (upE & ORF1a) real-time PCR Kit (PowerChek MERS assay; Kogene Biotech, Korea) a one-step rRT-PCR assay for the qualitative detection of MERS-CoV.
Methods: We evaluated PowerChek MERS assay performance in comparison with nested RT-PCR and sequencing of the RNA-dependent RNA polymerase (RdRp) and N genes. To evaluate diagnostic sensitivity and specificity, 100 clinical specimens (50 positive and 50 negative for MERS-CoV) were simultaneously tested by using the PowerChek MERS and sequencing assays. Assay performance, including limit of detection and precision, was evaluated in vitro by using MERS-CoV RNA transcripts. Analytical specificity was evaluated with a diverse collection of 16 respiratory virus–positive clinical specimens and 14 respiratory bacterial isolates.
Results: The 95% limits of detection of the PowerChek MERS assay for the upE and the open rading frame (ORF)1a were 16.2 copies/μL and 8.2 copies/μL, respectively. No cross-reactivity was observed. The diagnostic sensitivity and specificity of the PowerChek MERS assay were both 100% (95% confidence interval, 91.1–100%).
Conclusions: The PowerChek MERS assay is a straightforward and accurate assay for detecting MERS-CoV RNA. The assay will be a useful tool for the rapid diagnosis of MERS and could prove especially important for MERS outbreak control.
목차
등록된 정보가 없습니다.
참고문헌 (17)
참고문헌 신청
Bibby DF / 2011 / Comparative evaluation of the Seegene Seeplex RV15 and real-time PCR for respiratory virus detection / J Med Virol 83 : 1469 ~ 1475
Centers for Disease Control and Prevention / / Interim guidelines for collecting, handling, and testing clinical specimens from patients under investigation (PUIs) for Middle East R
Centers for Disease Control and Prevention / / Novel coronavirus 2012 real-time RT-PCR assay
Chan JF / 2015 / Development and evaluation of novel real-time reverse transcription-PCR assays with locked nucleic acid probes targeting leader sequences of human-pathogenic
Chan JF / 2015 / Middle East respiratory syndrome coronavirus: another zoonotic betacoronavirus causing SARS-like disease / Clin Microbiol Rev 28 : 465 ~ 522
Centers for Disease Control and Prevention / / Interim guidelines for collecting, handling, and testing clinical specimens from patients under investigation (PUIs) for Middle East R
Centers for Disease Control and Prevention / / Novel coronavirus 2012 real-time RT-PCR assay
Chan JF / 2015 / Development and evaluation of novel real-time reverse transcription-PCR assays with locked nucleic acid probes targeting leader sequences of human-pathogenic
Chan JF / 2015 / Middle East respiratory syndrome coronavirus: another zoonotic betacoronavirus causing SARS-like disease / Clin Microbiol Rev 28 : 465 ~ 522
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Analytical and Clinical Validation of Six Commercial Middle East Respiratory Syndrome Coronavirus RNA Detection Kits Based on Real-Time Reverse-Transcription PCR
Annals of Laboratory Medicine
2016 .01
-
MERS-CoV Infection in a Pregnant Woman in Korea
Journal of Korean Medical Science
2017 .01
-
Antiviral Treatment Guidelines for Middle East Respiratory Syndrome
Infection and Chemotherapy
2015 .01
-
Importance of Specimen Type and Quality in Diagnosing Middle East Respiratory Syndrome
Annals of Laboratory Medicine
2017 .01
-
External Quality Assessment of MERS-CoV Molecular Diagnostics During the 2015 Korean Outbreak
Annals of Laboratory Medicine
2016 .01
-
Guidelines for the Laboratory Diagnosis of Middle East Respiratory Syndrome Coronavirus in Korea
Infection and Chemotherapy
2016 .01
-
간호대학생의 중동호흡기증후군에 대한 지식, 태도 및 예방행위 실천 간의 관계
기초간호자연과학회지
2018 .01
-
Middle East Respiratory Syndrome Coronavirus Infection in Children
Pediatric Infection and Vaccine
2015 .01
-
Outbreaks of Middle East Respiratory Syndrome in Two Hospitals Initiated by a Single Patient in Daejeon, South Korea
Infection and Chemotherapy
2016 .01
-
Current Advances in the Development of Vaccines and Therapeutic Agents Against MERS-coV
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2015 .12
-
Korean Society for Laboratory Medicine Practice Guidelines for the Molecular Diagnosis of Middle East Respiratory Syndrome During an Outbreak in Korea in 2015
Annals of Laboratory Medicine
2016 .01
-
Immunogenicity of replication-defective recombinant vesicular stomatitis virus expressing Middle East respiratory syndrome coronavirus spike protein in mice
예방수의학회지
2021 .01
-
메르스(Middle East Respiratory Syndrome)의 유행이 의사의 정신건강에 미치는 영향 : 확진자 발생 병원 근무 여부, 메르스 진료 참여 여부에 따른 비교
신경정신의학
2017 .02
-
The Same Middle East Respiratory Syndrome- Coronavirus (MERS-CoV) yet Different Outbreak Patterns and Public Health Impacts on the Far East Expert Opinion from the Rapid Response Team of the Republic of Korea
Infection and Chemotherapy
2015 .01
-
Clinical Presentation and Outcomes of Middle East Respiratory Syndrome in the Republic of Korea
Infection and Chemotherapy
2016 .01
-
Collaborative Intervention of Middle East Respiratory Syndrome: Rapid Response Team
Infection and Chemotherapy
2016 .01
-
Development of transgenic mouse model for middle east respiratory syndrome coronavirus(MERS-CoV) infection
한국실험동물학회 학술발표대회 논문집
2019 .07
-
Considerations Left behind Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Outbreaks in Republic of Korea
대한폐경학회지
2015 .01
-
Urgent Call for Research on Middle East Respiratory Syndrome (MERS) in Korea
예방의학회지
2015 .01
-
2015년 한국의 중동호흡기증후군 유행 역학과 정책 과제
보건교육건강증진학회지
2015 .09
이 논문의 저자 정보
최근 본 자료
댓글(0)
0