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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 314 - 319 (6page)
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초록· 키워드
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Background : Mycobacterium tuberculosis is one of the most clinically significant infectious agents. Especially during mass outbreaks, accurate identification and monitoring are required. The proportion of
Beijing family members is very high among infecting strains, and spoligotyping is not suitable for strain typing. Therefore, we studied the homogeneity of isolates using the mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR) method and identified its utility for carrying out
molecular epidemiologic analysis.
Methods : Eighty-one clinical M. tuberculosis isolates that had previously been analyzed by spoligotyping were used in this study. We used the 12 standard MIRU loci and further four exact tandem repeat (ETR) loci (ETR-A, -B, -C, and -F). Four strains each of randomly selected Beijing and Beijinglike
families were subjected to IS6110- restriction fragment length polymorphism analysis.
Results : All 81 samples showed amplification products of all VNTR loci, and all of them showed differences in at least one locus. The calculation of the Hunter-Gaston diversity index (HGDI) for MIRU-VNTR gave the value of 0.965. Discriminatory index in the six loci (MIRU-10, -16, -26, -31, -39,
and ETR-F) were found to be highly discriminated (HGDI >0.6). Beijing and Beijing-like family isolates were discriminated into different MIRU-VNTR types.
Conclusions : MIRU-VNTR analysis by using well-selected loci can be useful in discriminating the clinical M. tuberculosis isolates in areas where the Beijing family is predominant.
목차
등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Alonso-Rodriguez N / 2008 / Evaluation of the new advanced 15-loci MIRU-VNTR genotyping tool in Mycobacterium tuberculosis molecular epidemiology studies / BMC Microbiol 8 :
Asgharzadeh M / 2007 / Current trends in molecular epidemiology studies of Mycobacterium tuberculosis / Biotechnol Mol Biol Rev 2 : 108 ~ 115
Bloom BR / 1992 / Tuberculosis: commentary on a reemergent killer / Science 257 : 1055 ~ 1064
Frothingham R / 1998 / Genetic diversity in the Mycobacterium tuberculosis complex based on variable numbers of tandem DNA repeats / Microbiology 144 : 1189 ~ 1196
Hunter PR / 1988 / Numerical index of the discriminatory ability of typing systems: an application of Simpson’s index of diversity / J Clin Microbiol 26 : 2465 ~ 2466
Asgharzadeh M / 2007 / Current trends in molecular epidemiology studies of Mycobacterium tuberculosis / Biotechnol Mol Biol Rev 2 : 108 ~ 115
Bloom BR / 1992 / Tuberculosis: commentary on a reemergent killer / Science 257 : 1055 ~ 1064
Frothingham R / 1998 / Genetic diversity in the Mycobacterium tuberculosis complex based on variable numbers of tandem DNA repeats / Microbiology 144 : 1189 ~ 1196
Hunter PR / 1988 / Numerical index of the discriminatory ability of typing systems: an application of Simpson’s index of diversity / J Clin Microbiol 26 : 2465 ~ 2466
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