지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 1,220 - 1,226 (7page)
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초록· 키워드
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Purpose: The Mycobacterium tuberculosis complex comprises M. tuberculosis, M. bovis, M. bovis bacillus Calmette-Guérin (BCG) and M. africanum, and causes tuberculosis in humans and animals. Identification of Mycobacterium spp. and M. tuberculosis complex to the species level is important for practical use in microbiological laboratories, in addition to optimal treatment and public health. Materials and Methods: A novel multiplex PCR assay targeting a conserved rpoB sequence in Mycobacteria spp., as well as regions of difference (RD) 1 and RD8, was developed and evaluated using 37 reference strains and 178 clinical isolates. Results: All mycobacterial strains produced a 518-bp product (rpoB), while other bacteria produced no product. Virulent M. tuberculosis complex strains, M. tuberculosis, M. bovis and M. africanum, produced a 254-bp product (RD1), while M. bovis BCG, M. microti and nontuberculous mycobacteria produced no RD1 region product. Additionally, M. tuberculosis and M. africanum produced a 150-bp product (RD8), while M. bovis and M. bovis BCG produced a 360-bp product (deleted form of RD8). M. microti and nontuberculous mycobacteria produced no RD8 region product. This assay identified all Mycobacterium spp. and all M. tuberculosis complex strains to the species level. Conclusion: The multiplex PCR assay of the present study could be implemented as a routine test in microbiology laboratories, and may contribute to more effective treatment and surveillance of tuberculosis stemming from the M. tuberculosis complex.
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참고문헌 (19)
참고문헌 신청
Butler WR / 1991 / Identification of mycobacteria by high-performance liquid chromatography / J Clin Microbiol 29 : 2468 ~ 2472
Dobner P / 1996 / Rapid identification of mycobacterial species by PCR amplification of hypervariable 16S rRNA gene promoter region / J Clin Microbiol 34 : 866 ~ 869
Ernst JD / 2007 / Genomics and the evolution, pathogenesis, and diagnosis of tuberculosis / J Clin Invest 117 : 1738 ~ 1745
Frota CC / 2004 / Genome structure in the vole bacillus, Mycobacterium microti, a member of the Mycobacterium tuberculosis complex with a low virulence for humans / Microbio
Frothingham R / 1995 / Differentiation of strains in Mycobacterium tuberculosis complex by DNA sequence polymorphisms, including rapid identification of M. bovis BCG / J Clin
Dobner P / 1996 / Rapid identification of mycobacterial species by PCR amplification of hypervariable 16S rRNA gene promoter region / J Clin Microbiol 34 : 866 ~ 869
Ernst JD / 2007 / Genomics and the evolution, pathogenesis, and diagnosis of tuberculosis / J Clin Invest 117 : 1738 ~ 1745
Frota CC / 2004 / Genome structure in the vole bacillus, Mycobacterium microti, a member of the Mycobacterium tuberculosis complex with a low virulence for humans / Microbio
Frothingham R / 1995 / Differentiation of strains in Mycobacterium tuberculosis complex by DNA sequence polymorphisms, including rapid identification of M. bovis BCG / J Clin
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