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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 130 - 135 (6page)
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초록· 키워드
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Background : Leukapheresis has commonly been used to obtain the cell products intended for
clinical cell therapy. Hypocalcemia related to citrate toxicity and some circulatory effects such as
hypovolemia and hypotension are well-known complications of leukapheresis. In this study, we analyzed
the gene expression profiles of peripheral blood mononuclear cells (PBMCs) obtained before
and after leukapheresis to determine if the hemodynamic changes can affect the gene expression
profiles of leukocytes.
Methods : PBMCs were isolated from EDTA blood from 5 healthy donors collected before and
immediately after apheresis. RNA was isolated, amplified, and analyzed using a cDNA microarray
with 17,500 genes. Hierarchical clustering analysis was performed to evaluate the differences of
gene expression profiling.
Results : Hierarchical clustering separated PBMCs from different donors with each other, but did
not separate PBMCs collected before and after leukapheresis. Comparison of gene expression by
PBMCs collected before and after leukapheresis found only 25 genes were differentially expressed
(15 were up-regulated and 10 were down-regulated after leukapheresis) (F-test, P<0.005). Stress
induced apoptosis-related genes, ANXA3, DEDD, and ATXN2L, and cytokine-related genes, IL13RA1
and IK, which were also related to stress, were up-regulated after leukapheresis. Genes involved in
DNA and protein binding, such as CLSTN3, LRBA, SATB2, and HSPA8, were down-regulated.
Conclusions : Leukapheresis had little effect on gene expression of PBMCs. Some genes showing
differences between before and after leukapheresis were mainly involved in stress-related reactions.
(Korean J Lab Med 2008;28:130-5)
목차
등록된 정보가 없습니다.
참고문헌 (19)
참고문헌 신청
Baechler EC / 2004 / Expression levels for many genes in human peripheral blood cells are highly sensitive to ex vivo incubation / Genes Immun 5 : 347 ~ 353
Brecher ME / 2005 / Technical manual / American Association of Blood Banks : 587 ~
Dauer M / 2003 / Mature dendritic cells derived from human monocytes within 48 hours: a novel strategy for dendritic cell differentiation from blood precursors / J Immunol 17
Eisen MB / 1998 / Cluster analysis and display of genome-wide expression patterns / Proc Natl Acad Sci USA 95 : 14863 ~ 14868
Felzmann T / 2003 / Monocyte enrichment from leukapharesis products for the generation of DCs by plastic adherence, or by positive or negative selection / Cytotherapy 5 : 391
Brecher ME / 2005 / Technical manual / American Association of Blood Banks : 587 ~
Dauer M / 2003 / Mature dendritic cells derived from human monocytes within 48 hours: a novel strategy for dendritic cell differentiation from blood precursors / J Immunol 17
Eisen MB / 1998 / Cluster analysis and display of genome-wide expression patterns / Proc Natl Acad Sci USA 95 : 14863 ~ 14868
Felzmann T / 2003 / Monocyte enrichment from leukapharesis products for the generation of DCs by plastic adherence, or by positive or negative selection / Cytotherapy 5 : 391
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