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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 353 - 361 (9page)
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초록· 키워드
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Background : Detection of antibodies to extractable nuclear antigens (ENAs) and dsDNA is needed for the diagnosis of and predicting prognosis in systemic autoimmune diseases. Recently introduced line immunoassay (LIA) has the advantage of detecting several autoantibodies simultaneously,
and we evaluated its usefulness in the diagnosis of autoimmune diseases in comparison with enzyme-linked immunosorbent assay (ELISA).
Methods : Samples were collected from 437 patients referred by rheumatologists. FANA (fluorescent antinuclear antibody) test and LIA for the detection of 13 different autoantibodies, including 6 ENAs and dsDNA were performed. LIA-positive samples for ENA or dsDNA antibodies were further
tested with ELISA. Final diagnosis was made by rheumatologists according to the diagnostic criteria.
Agreement of results between LIA and ELISA was analyzed in 53 selected patients with systemic autoimmune diseases.
Results : The LIA detected antibodies to ENA and dsDNA in 118 and 22 patients, respectively, and ELISA detected 70.3% (83/118) and 45.5% (10/22) of LIA positive samples. Especially, 60.2% (71/118) of patients with positive ENA antibody on LIA was diagnosed as systemic autoimmune
diseases. Patients having strong FANA titer and homogenous/speckled pattern showed higher prevalence of autoantibodies, but a small proportion of FANA negative patients also showed positive reactivity (LIA 10.8%, ELISA 5.2%). LIA showed a good agreement with ELISA for the anti-ENA
antibodies (≥80%), and a lower agreement for the anti-dsDNA antibody (67.9%).
Conclusions : LIA detecting several autoantibodies simultaneously might replace ELISA for anti-ENA antibodies, but not for anti-dsDNA antibodies. When LIA is performed considering clinical manifestations and FANA, it could contribute to the diagnosis of systemic autoimmune disease. (Korean J Lab Med 2008;28:353-61)
목차
등록된 정보가 없습니다.
참고문헌 (16)
참고문헌 신청
Arbuckle MR / 2003 / Development of autoantibodies before the clinical onset of systemic lupus eythematosus / N Eng J Med 349 : 1526 ~ 1533
Bossuyt X / 2005 / Antibodies to extractable nuclear antigens in antinuclear antibody-negative samples / Clin Chem 51 : 2426 ~ 2427
Damoiseaux J / 2005 / Evaluation of a novel line-blot immunoassay for the detection of antibodies to extractable nuclear antigens / Ann N Y Acad Sci 1050 : 340 ~ 347
Damoiseaux JG / 2006 / From ANA to ENA: how to proceed? / Autoimmun Rev 5 : 10 ~ 17
Delpech A / 1993 / Antibodies to Sm, RNP and SSB detected by solid-phase ELISAs using recombinant antigens: a comparison study with counter immunoelectrophoresis and immunobl
Bossuyt X / 2005 / Antibodies to extractable nuclear antigens in antinuclear antibody-negative samples / Clin Chem 51 : 2426 ~ 2427
Damoiseaux J / 2005 / Evaluation of a novel line-blot immunoassay for the detection of antibodies to extractable nuclear antigens / Ann N Y Acad Sci 1050 : 340 ~ 347
Damoiseaux JG / 2006 / From ANA to ENA: how to proceed? / Autoimmun Rev 5 : 10 ~ 17
Delpech A / 1993 / Antibodies to Sm, RNP and SSB detected by solid-phase ELISAs using recombinant antigens: a comparison study with counter immunoelectrophoresis and immunobl
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