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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 24 - 33 (10page)
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초록· 키워드
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Background : Infections caused by mycobacteria have been significantly increasing. Due to the
difficulty of making a decision about the pathogenicity of mycobacteria, species-level identification is
very important for patients’ diagnosis and treatment. The purpose of this study was to identify mycobacteria
species using a high performance liquid chromatography (HPLC) method and to provide an initial
database for the distribution of mycobacteria in Korea.
Methods : Acid fast bacteria isolated from 3,107 clinical specimens were identified by mycolic
acid analysis using HPLC. The HPLC patterns were compared with those of standard mycobacteria
species.
Results : The HPLC patterns were divided into single, double, and triple cluster groups, each group
comprising 9, 20, and 4 species, respectively. Mycobacteria and non-tuberculous mycobacteria
(NTM) were identifies by HPLC at the rates of 99.5% and 95.6%, respectively. NTM was isolated in
12.4% of the mycobacteria positive specimens. This study also found that there were 20 different
NTM species with the distribution of each species ranging from 0.3% to 15.9% of the total NTM.
While the rate of NTM has been increasing in Korea, M. avium-intracellulare, M. fortuitum, and M.
chelonae are relatively decreasing, and M. kansasii and M. gordonae are relatively increasing.
Conclusions : HPLC method was highly discriminative for the identification of NTM in clinical specimens.
(Korean J Lab Med 2008;28:24-33)
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등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
/ 1997 / Diagnosis and treatment of disease caused by nontuberculous mycobacteria. This official statement of the American Thoracic Society was approved by the Board of Directors, Mar
/ 2005 / National Committee for Clinical Laboratory Standards. Molecular diagnostic methods for infectious diseases. Proposed guideline, 2nd ed. MM3-P2. Wayne, Pa: NCCLS, 2005
Burman, W.J. / 2000 / Review of false positive cultures for Mycobacterium tuberculosis and recommendations for avoiding unnecessary treatment / Clin Infect Dis 31 : 1390 ~ 1
Butler, W.R. / 1991 / Identification of mycobacteria by high-performance liquid chromatography / J Clin Microbiol 29 : 2468 ~ 2472
Butler, W.R. / 1996 / Standardized method for HPLC identification of mycobacteri
/ 2005 / National Committee for Clinical Laboratory Standards. Molecular diagnostic methods for infectious diseases. Proposed guideline, 2nd ed. MM3-P2. Wayne, Pa: NCCLS, 2005
Burman, W.J. / 2000 / Review of false positive cultures for Mycobacterium tuberculosis and recommendations for avoiding unnecessary treatment / Clin Infect Dis 31 : 1390 ~ 1
Butler, W.R. / 1991 / Identification of mycobacteria by high-performance liquid chromatography / J Clin Microbiol 29 : 2468 ~ 2472
Butler, W.R. / 1996 / Standardized method for HPLC identification of mycobacteri
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