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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 239 - 243 (5page)
이용수
초록· 키워드
오류제보하기
Background : Commercial kits of PCR method are widely used in HLA-B27 typing; however, their
cost is relatively high. In this study, we evaluated the utility of an in-house PCR method by comparing
it with that of a commercial kit.
Methods : HLA-B27 typing was done in 188 patients by using two PCR methods, AbsoluteTM HLAB27
PCR kit (Biosewoom, Korea) and an in-house PCR method. The primers used in the in-house
method were prepared by Bioneer (Korea). Both PCR tests were done by Gene Amp PCR System
9600 (Perkin-Elmer Centus Corp., USA).
Results : The commercial kit and in-house PCR showed 100% concordance rate with each other
in HLA-B27 typing. Of 188 patients tested 72 (38.3%) were positive and 116 (61.7%) were negative
by the both tests. Of 62 patients with ankylosing spondylitis, 50 were positive (80.7%).
Conclusions : The in-house PCR is a reliable and cost-effective method and can replace or supplement
commercial kits for HLA-B27 typing. (Korean J Lab Med 2008;28:239-43)
목차
등록된 정보가 없습니다.
참고문헌 (11)
참고문헌 신청
Bae JS / 2000 / Comparison of HLA-B27 typing methods- PCR-SSP, microlymphocytotoxicity, and flow cytometry- / Korean J Clin Pathol 20 : 198 ~ 203
Bonnaud G / 1999 / Optimisation of HLA-B27 testing by association of flow cytometry and DNA typing / Clin Rheumatol 18 : 23 ~ 27
Bunce M / 1995 / Phototyping: comprehensive DNA typing for HLA-A, B, C, DRB1, DRB3, DRB4, DRB5 & DQB1 by PCR with 144 primer mixes utilizing sequence-specific primers (PC
Downing J / 2006 / A high-resolution polymerase chain reaction-sequence-specific primer HLA-B*27 typing set and its application in routine HLAB27 testing / Genet Test 10 : 98
Inaoui R / 2004 / Outcome of patients with undifferentiated chronic monoarthritis: retrospective study of 46 cases / Joint Bone Spine 71 : 209 ~ 213
Bonnaud G / 1999 / Optimisation of HLA-B27 testing by association of flow cytometry and DNA typing / Clin Rheumatol 18 : 23 ~ 27
Bunce M / 1995 / Phototyping: comprehensive DNA typing for HLA-A, B, C, DRB1, DRB3, DRB4, DRB5 & DQB1 by PCR with 144 primer mixes utilizing sequence-specific primers (PC
Downing J / 2006 / A high-resolution polymerase chain reaction-sequence-specific primer HLA-B*27 typing set and its application in routine HLAB27 testing / Genet Test 10 : 98
Inaoui R / 2004 / Outcome of patients with undifferentiated chronic monoarthritis: retrospective study of 46 cases / Joint Bone Spine 71 : 209 ~ 213
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