지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 301 - 306 (6page)
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초록· 키워드
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Purpose: PCR is widely used for rapidly and accurately detecting Mycobacterium species. The purpose of this study was to assess the diagnostic performance of three real-time PCR kits and evaluate the concordance with two older PCR methods.
Materials and Methods: Using 128 samples, the five PCR methods were assessed,including an in-house PCR protocol, the COBAS Amplicor MTB, the COBAS TaqMan MTB, the AdvanSure TB/NTM real-time PCR, and the Real-Q M. tuberculosis kit. The discrepant results were further examined by DNA sequencing and using the AdvanSure Mycobacteria Genotyping Chip for complete analysis. Results: For Mycobacterium tuberculosis (MTB) detection, all five kits showed 100% matching results (positive; N = 11 and negative; N = 80). In non-tuberculous mycobacterium (NTM) discrimination, the AdvanSure yielded two true-positive outcomes from M. intracellulare and one false positive outcome, while the Real-Q resulted in one true-positive outcome and one false negative outcome for each case and another false negative result using the provided DNA samples. Conclusion:Real-time PCR, yielded results that were comparable to those of the older PCR methods for detecting MTB. However, there were disagreements among the applied kits in regard to the sample test results for detecting NTM. Therefore, we recommend that additional confirmatory measures such as DNA sequencing should be implemented in such cases, and further research with using a larger numbers of samples is warranted to improve the detection of NTM.
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참고문헌 (16)
참고문헌 신청
Baba K / 2008 / Real-time quantitative PCR in the diagnosis of tuberculosis in formalin-fixed paraffin-embedded pleural tissue in patients from a high HIV endemic area / Diag
Choi YM / 1999 / Detection of Mycobacterium tuberculosis in sputum by using polymerase chain reaction / Korean J Clin Microbiol 2 : 144 ~ 151
Flores E / 2009 / Real-time PCR with internal amplification control for detecting tuberculosis: method design and validation / APMIS 117 : 592 ~ 597
Goessens WH / 2005 / Comparison of the COBAS AMPLICOR MTB and BDProbeTec ET assays for detection of Mycobacterium tuberculosis in respiratory specimens / J Clin Microbiol 43
Lee H / 2000 / Species identification of mycobacteria by PCR-restriction fragment length polymorphism of the rpoB gene / J Clin Microbiol 38 : 2966 ~ 2971
Choi YM / 1999 / Detection of Mycobacterium tuberculosis in sputum by using polymerase chain reaction / Korean J Clin Microbiol 2 : 144 ~ 151
Flores E / 2009 / Real-time PCR with internal amplification control for detecting tuberculosis: method design and validation / APMIS 117 : 592 ~ 597
Goessens WH / 2005 / Comparison of the COBAS AMPLICOR MTB and BDProbeTec ET assays for detection of Mycobacterium tuberculosis in respiratory specimens / J Clin Microbiol 43
Lee H / 2000 / Species identification of mycobacteria by PCR-restriction fragment length polymorphism of the rpoB gene / J Clin Microbiol 38 : 2966 ~ 2971
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