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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 79 - 91 (13page)
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Purpose Genetically engineered stem cells may be advantageous for gene therapy against various human cancers due to their inherent tumor-tropic properties. In this study, genetically engineered human neural stem cells (HB1.F3) expressing Escherichia coli cytosine deaminase (CD) (HB1.F3.CD) and human interferon-β (IFN-β) (HB1.F3.CD.IFN-β) were employed against lymph node–derived metastatic colorectal adenocarcinoma.
Materials and Methods CD can convert a prodrug, 5-fluorocytosine (5-FC), to active 5-fluorouracil, which inhibits tumor growth through the inhibition of DNA synthesis, while IFN-β also strongly inhibits tumor growth by inducing the apoptotic process. In reverse transcription polymerase chain reaction analysis, we confirmed that HB1.F3.CD cells expressed the CD gene and HB1.F3.CD.IFN-β cells expressed both CD and IFN-β genes.
Results In results of a modified trans-well migration assay, HB1.F3.CD and HB1.F3.CD.IFN-β cells selectively migrated toward SW-620, human lymph node–derived metastatic colorectal adenocarcinoma cells. The viability of SW-620 cells was significantly reduced when co-cultured with HB1.F3.CD or HB1.F3.CD.IFN-β cells in the presence of 5-FC. In addition, it was found that the tumor-tropic properties of these engineered human neural stem cells (hNSCs) were attributed to chemoattractant molecules including stromal cell-derived factor 1, c-Kit, urokinase receptor, urokinase-type plasminogen activator, and C-C chemokine receptor type 2 secreted by SW-620 cells. In a xenograft mouse model, treatment with hNSC resulted in significantly inhibited growth of the tumor mass without virulent effects on the animals.
Conclusion The current results indicate that engineered hNSCs and a prodrug treatment inhibited the growth of SW-620 cells. Therefore, hNSC therapy may be a clinically effective tool for the treatment of lymph node metastatic colorectal cancer.
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참고문헌 (22)
참고문헌 신청
Bockelman C / 2015 / Risk of recurrence in patients with colon cancer stage II and III : a systematic review and meta-analysis of recent literature / Acta Oncol 54 : 5 ~ 16
Brenner H / 2014 / Colorectal cancer / Lancet 383 : 1490 ~ 1502
Dhar DK / 2005 / Metastatic lymph node size and colorectal cancer prognosis / J Am Coll Surg 200 : 20 ~ 28
Freytag SO / 2002 / Phase I study of replication-competent adenovirus-mediated double suicide gene therapy for the treatment of locally recurrent prostate cancer / Cancer Res
Fulmes M / 2005 / Cancer biology and necrotic changes in metastatic lymph nodes and survival of colon cancer patients / Am J Surg 189 : 364 ~ 368
Brenner H / 2014 / Colorectal cancer / Lancet 383 : 1490 ~ 1502
Dhar DK / 2005 / Metastatic lymph node size and colorectal cancer prognosis / J Am Coll Surg 200 : 20 ~ 28
Freytag SO / 2002 / Phase I study of replication-competent adenovirus-mediated double suicide gene therapy for the treatment of locally recurrent prostate cancer / Cancer Res
Fulmes M / 2005 / Cancer biology and necrotic changes in metastatic lymph nodes and survival of colon cancer patients / Am J Surg 189 : 364 ~ 368
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