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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한소아알레르기호흡기학회 Allergy Asthma & Respiratory Diseases Allergy Asthma & Respiratory Diseases 제18권 제1호
- 발행연도
- 2008.1
- 수록면
- 37 - 45 (9page)
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초록· 키워드
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Purpose:The aim of this study was to determine the allergenicity of soybean by fermentation.
Methods:Non-fermented soybean, fermented soybean by Bacillus subtilis KFCC 11293 and 3-step fermented soybean by Lactococcus lactis subsp. lactis IFO 12007 and Aspergillus oryzae, B. subtilis KFCC 11293 were extracted with phosphate buffered saline (PBS). In order to detect soybean-specific IgE, we performed SDS-PAGE and IgE immunoblot analysis by using 3 kinds of soybean extracts and sera from 9 patients with atopic dermatitis. All patients were sensitive to soybean, which were confirmed by CAP-FEIA.
Results:SDS-PAGE of non-fermented soybeans showed many bands, whereas only peptides of less than 15 kDa were found in fermented soybeans. IgE immunoblot analysis of fermented soybeans failed to detect specific IgE which were seen in non-fermented soybeans.
Conclusion:Fermentation could reduce the allergenicity of soybeans by efficiently degrading antigenic proteins in soybeans. However, there was no significant difference between fermentation by B. subtilis and the 3-step fermentation.
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참고문헌 (26)
참고문헌 신청
[학
Ahn KM / 2003 / Prevalence of soy protein hypersensitivity in cow's milk protein-sensitive children in Korea / J Korean Med Sci 18 : 473 ~ 477
Bock SA / 1987 / Prospective appraisal of complaints of adverse reactions to foods in children during the first 3 years of life / Pediatrics 79 : 683 ~ 688
Errahali Y / 2002 / Allergen in soy oils / Allergy 57 : 648 ~ 649
Foucard T / 1999 / A study on severe food reactions in Sweden is soy protein an underestimated cause of food anaphylaxis / Allergy 54 : 261 ~ 265
Ahn KM / 2003 / Prevalence of soy protein hypersensitivity in cow's milk protein-sensitive children in Korea / J Korean Med Sci 18 : 473 ~ 477
Bock SA / 1987 / Prospective appraisal of complaints of adverse reactions to foods in children during the first 3 years of life / Pediatrics 79 : 683 ~ 688
Errahali Y / 2002 / Allergen in soy oils / Allergy 57 : 648 ~ 649
Foucard T / 1999 / A study on severe food reactions in Sweden is soy protein an underestimated cause of food anaphylaxis / Allergy 54 : 261 ~ 265
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