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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한병리학회 Journal of Pathology and Translational Medicine Journal of Pathology and Translational Medicine 제44권 제3호
- 발행연도
- 2010.1
- 수록면
- 259 - 266 (8page)
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초록· 키워드
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Background : To determine the most effective method for detecting mycobacteria in formalin-fixed paraffin-embedded (FFPE) tissue, we compared the results of Ziehl-Neelsen stain (ZNS) and mycobacterial culture with those of polymerase chain reaction (PCR) and real-time quantitative PCR (RQ-PCR). Methods : We analyzed 54 cases diagnosed as chronic granulomatous inflammation. In all cases, ZNS and nested PCR using three different primers, IS6110, Mpb64 and IS6110/Rpoβ were done. RQ-PCR with the IS6110/Rpoβ primer was done in 51 cases. Results : Mycobacteria were identified by ZNS in 15/54 (27.8%) cases. RQ-PCR had the highest sensitivity (80.0%) compared to PCR with IS6110 (73.3%), Mpb64 (60.0%) and IS6110/Rpoβ (73.3%). Specificity was higher in all PCR experiments (79.5-82.1%) than in RQ-PCR (69.4%) experiments. The false negative rate was lowest for RQ-PCR (20.0%) than for PCR with IS6110 (26.7%), Mpb64 (40.0%) and IS6110/Rpoβ (26.7%). The false positive rate was highest for RQ-PCR (30.6%) compared to PCR with IS6110 (20.5%), Mpb64 (17.9%) and IS6110/Rpoβ (20.5%). Conclusions : RQ-PCR had the highest sensitivity, and the lowest false negative rate, but it also had a higher false positive rate than PCR for detection of mycobacteria in FFPE tissues.
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참고문헌 (24)
참고문헌 신청
Ahmed A / 2009 / Development and validation of a real-time PCR for detection of pathogenic leptospira species in clinical materials / PLoS One 4 : 7093 ~
Baba K / 2008 / Real-time quantitative PCR in the diagnosis of tuberculosis in formalin-fixed paraffin-embedded pleural tissue in patients from a high HIV endemic area / Diag
Beqaj SH / 2007 / Use of the real-time PCR assay in conjunction with MagNA Pure for the detection of mycobacterial DNA from fixed specimens / Diagn Mol Pathol 16 : 169 ~ 173
Brugiere O / 1997 / Diagnosis of smear-negative pulmonary tuberculosis using sequence capture polymerase chain reaction / Am J Respir Crit Care Med 155 : 1478 ~ 1481
Charles M / 2006 / Tuberculosis and HIV:implications in the developing world / Curr HIV/AIDS Rep 3 : 139 ~ 144
Baba K / 2008 / Real-time quantitative PCR in the diagnosis of tuberculosis in formalin-fixed paraffin-embedded pleural tissue in patients from a high HIV endemic area / Diag
Beqaj SH / 2007 / Use of the real-time PCR assay in conjunction with MagNA Pure for the detection of mycobacterial DNA from fixed specimens / Diagn Mol Pathol 16 : 169 ~ 173
Brugiere O / 1997 / Diagnosis of smear-negative pulmonary tuberculosis using sequence capture polymerase chain reaction / Am J Respir Crit Care Med 155 : 1478 ~ 1481
Charles M / 2006 / Tuberculosis and HIV:implications in the developing world / Curr HIV/AIDS Rep 3 : 139 ~ 144
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