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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 43 - 48 (6page)
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초록· 키워드
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It is a serious situation that the farmers' incomehas gradually decreased due to the decline of productivity offruit trees infected with viroids. It has been known thatPersimmon viroid (PVd) and Citrus viroid (CVd) are economicallyimportant viroids that can infected persimmon. In this study,the incidence of CVd and PVd in ‘Fuyu’ persimmon wereidentified as 41% and 34% in JeollaNam-do, respectively.
The collected persimmon samples infected by both PVd andCVd were used for testing efficiency of the viroid inactivationmethods. The samples were subjected to single treatment ofthe heat treatment (37°C), cold treatment (4°C), or antiviralagent treatment (Ribavirin), and double treatment of combinationsof the three methods. Viroid inactivation efficiency wasconfirmed through RT-PCR. In the case of the samplessubjected to cold treatment for 4 weeks, the viroid inactivationefficiency was most significantly high as 67% against thesurvival rate of 100%. In addition, in the case of the samplestreated for 2 weeks with the antiviral agents and cold treatment, the viroid inactivation rate was similar to that ofthe cold treatment. In conclusion, the cold treatment showedthe highest viroid inactivation efficiency, and this result willprovide valuable information for production of viroid-freepersimmon.
목차
등록된 정보가 없습니다.
참고문헌 (14)
참고문헌 신청
Campbell AI / 1962 / Apple virus inactivation by heat therapy and tip propagation / Nature 195 : 520 ~
El-Dougdoug KA / 2010 / Elimination of Hop stunt viroid (HSVd) from infected peach and pear plants using cold therapy and chemotherapy / Aust. J. Basic Appl. Sci 4 : 54 ~ 60
Feng C / 2013 / Production of pathogen-free horticultural crops by cryotherapy of in vitro-grown shoot tips / Methods Mol. Biol 994 : 463 ~ 482
Gambino G / 2008 / A Rapid and effective method for RNA extraction from different tissues of grapevine and other woody plants / Phytochem. Anal 19 : 520 ~ 525
Hansen A / 1985 / Elimination of apple chlorotic leaf spot virus from apple shoot cultures by ribavirin / Plant Dis 69 : 134 ~ 135
El-Dougdoug KA / 2010 / Elimination of Hop stunt viroid (HSVd) from infected peach and pear plants using cold therapy and chemotherapy / Aust. J. Basic Appl. Sci 4 : 54 ~ 60
Feng C / 2013 / Production of pathogen-free horticultural crops by cryotherapy of in vitro-grown shoot tips / Methods Mol. Biol 994 : 463 ~ 482
Gambino G / 2008 / A Rapid and effective method for RNA extraction from different tissues of grapevine and other woody plants / Phytochem. Anal 19 : 520 ~ 525
Hansen A / 1985 / Elimination of apple chlorotic leaf spot virus from apple shoot cultures by ribavirin / Plant Dis 69 : 134 ~ 135
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