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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 187 - 195 (9page)
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Apples are produced using a high-density plantation system using M.9 rootstock in Korea. However, the virus infection rate of apples tends to be high because the rootstocks produced from virus-infected mother stocks were widely used and vegeta?tive propagation based on the grafting is common with scion cultivars. The most efective way to control plant virus and viroid depends on conducting a massively rapid and precise diagnostic inspection. Therefore, this study aims to develop a real-time PCR assay that can be precisely and inexpensively tested for ACLSV and ASSVd. To expand the detection range, we developed a singleplex real-time PCR method targeting the conserved region of ACLSV and ASSVd, respectively. The detection methods using two kinds of fuorescent chemical dye, SYBR Green I and TaqMan probe, have been developed in this study. Comparing these methods developed with two dyes, the assay using SYBR Green I was efcient for detection as much as the method using TaqMan probe and faster. The feld test demonstrated real-time detecting methods developed using SYBR Green I dye were applicable to reliable diagnosis and quantifcation for virus and viroid in quarantine felds.
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참고문헌 (47)
참고문헌 신청
Al-Rwahnih M / 2004 / Molecular variability of Apple chlorotic leaf spot virus in different hosts and geographical regions / J Plant Pathol 86:117~122
Cambell A / 1976 / Experiments with dapple apple virus / Acta Hortic 67:261~264
Chae JH / 2016 / An improvement plan for nursery tree production and distribution
CHEONG EUN JU / 2012 / Biotechnological approaches for improvement and conservation of Prunus species / Plant Biotechnology Reports 6(1):17~28
조인숙 / 2010 / 국내에서 발생하는 배나무 바이러스병 / 식물병 연구 16(3):326~330
Cambell A / 1976 / Experiments with dapple apple virus / Acta Hortic 67:261~264
Chae JH / 2016 / An improvement plan for nursery tree production and distribution
CHEONG EUN JU / 2012 / Biotechnological approaches for improvement and conservation of Prunus species / Plant Biotechnology Reports 6(1):17~28
조인숙 / 2010 / 국내에서 발생하는 배나무 바이러스병 / 식물병 연구 16(3):326~330
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