지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 462 - 471 (10page)
이용수
초록· 키워드
오류제보하기
Thiamine pyrophosphate (TPP), the active form of thiamine is a cofactor for enzymes involved in central metabolism pathways. However, it is also known to have a role as a stress signaling molecule in response to environmental changes. Anabaena sp. and N. oculata are microorganisms which are abundantly found in Malaysia’s freshwater and marine ecosystem. However, not much studies have been done especially in regards to thiamine biosynthesis. This work aimed to amplify of gene transcripts coding for thiamine biosynthesis enzymes besides looking at the expression of thiamine biosynthesis genes upon stress application. Various stress inducers were applied to the cultures and RNA was extracted at different time points. The first two genes, ThiC and ThiG/Thi4 encoding enzymes of the pyrimidine and thiazole branch respectively in the thiamine biosynthesis pathway were identified and amplified. The expression of the genes were analysed via RT-PCR and the intensity of bands were analysed using ImageJ software. The results showed up to 4-fold increase in the expression of ThiC and ThiG gene transcript as compared to control sample in Anabaena sp.
ThiC gene in N. oculata showed an expression of 6-fold higher as compared to control sample. In conclusion, stresses induced the expression of the gene coding for one of the most important enzymes in thiamine biosynthesis pathway. This is an agreement with the hypothesis that overexpression of thiamine is crucial in assisting plants to combat abiotic stresses.
목차
등록된 정보가 없습니다.
참고문헌 (59)
참고문헌 신청
Haas RH / 1988 / Thiamine and the brain / Annual reviews 8:483~515
Huertas MJ / 2014 / Metals in cyanobacteria: analysis of the copper, nickel, cobalt and arsenic homeostasis mechanisms / Life 4:865~886
Hughes EO / 1958 / Toxicity of a unialgal culture of Microcystis aeruginosa / Canadian J Microb 4:225~236
Kagan, ML / 2015 / Safety assessment of the microalgae Nannochloropsis oculata / Toxicology report 2:617~623
Kaneko T / 2001 / Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120 / DNA research 8:205~213
Huertas MJ / 2014 / Metals in cyanobacteria: analysis of the copper, nickel, cobalt and arsenic homeostasis mechanisms / Life 4:865~886
Hughes EO / 1958 / Toxicity of a unialgal culture of Microcystis aeruginosa / Canadian J Microb 4:225~236
Kagan, ML / 2015 / Safety assessment of the microalgae Nannochloropsis oculata / Toxicology report 2:617~623
Kaneko T / 2001 / Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120 / DNA research 8:205~213
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Determination of thiamine and niacin contents in meat processed foods
한국식품영양과학회 학술대회발표집
2022 .10
-
Thiamine, riboflavin and niacin contents in fruits
한국식품영양과학회 학술대회발표집
2023 .10
-
Contents of thiamine, riboflavin, and niacin in sprouted vegetables
한국식품영양과학회 학술대회발표집
2022 .10
-
Evaluation of thiamine and niacin in processed seafood
한국식품영양과학회 학술대회발표집
2021 .10
-
Determination of thiamine, riboflavin and niacin contents in 23 kinds of late-night snack foods
한국식품영양과학회 학술대회발표집
2023 .10
-
Dietary thiamine requirement for Pacific white shrimp (Litopenaeus vannamei)
한국수산과학회 양식분과 학술대회
2022 .10
-
국내 육성 잡곡에 함유된 Thiamine, Riboflavin 및 Niacin 함량
한국식품영양과학회지
2021 .12
-
Toward to Comparative Analysis of Plant Stress-responsive Co-expression Network Using RNA-seq in Capsicum annuum L.
한국원예학회 학술발표요지
2021 .10
-
Transcriptome profiling of the coffee (C. arabica L.) seedlings under salt stress condition
Journal of Plant Biotechnology
2018 .01
-
Characterization of Ethylene Biosynthesis and Signaling Genes in Cucumber
한국원예학회 학술발표요지
2016 .05
-
Transcriptome analysis of Korean fir (Abies koreana) in response to elevated carbon dioxide and high temperature
Plant Biotechnology Reports
2019 .01
-
Gene co-expression network reconstruction: a review on computational methods for inferring functional information from plant-based expression data
Plant Biotechnology Reports
2017 .04
-
국내 육성 신품종 현미 및 백미에 함유된 수용성 비타민 티아민, 리보플라빈 및 니아신 함량
한국식품영양과학회지
2020 .09
-
Differential expression of ginsenoside biosynthesis-related genes at an early developmental stage of Panax vietnamensis
Plant Biotechnology Reports
2022 .04
-
Light and Transient Expression of Carotenogenic Genes Induces Carotenoid Biosynthesis-related Gene Expression and Pigment Content Accumulation in Apple ‘RubyS’ Fruit
한국원예학회 학술발표요지
2022 .05
-
EFFECT OF DIETARY TOCOPHEROL, THIAMINE, SELENIUM, AND METHIONINE ON MERCURY EXCRETION AND BIOCHEMICAL RESPONSES IN MERCURY EXPOSED OLIVE FLOUNDER Paralichthys olivaceus
한국수산과학회 양식분과 학술대회
2015 .05
-
Transcriptomic analysis of endoplasmic reticulum stress in roots of grapevine rootstock
Plant Biotechnology Reports
2021 .10
-
베트남 식품의 티아민, 리보플라빈 및 니아신 함량 평가
한국식품영양과학회지
2023 .01
-
De novo gene set assembly of the transcriptome of diploid, oilseed-crop species Perilla citriodora
Journal of Plant Biotechnology
2016 .01
-
Screening of Multiple Abiotic Stress-Induced Genes in Italian Ryegrass leaves
한국초지조사료학회지
2018 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0