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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 55 - 60 (6page)
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This study investigated the in vitro symbiotic seed germination of the achlorophyllous orchid, Gastrodia elata, using a new Mycena species. A leaf-disc (2x2 cm) of Quercus accutissima was inoculated with either of the two fungal species, NIFOS101 (NCBI accession number KY449288) or KFRI1212 (HQ662845), following which it was placed on water agar medium, prior to sowing seeds of G. elata. After 20 days of symbiotic culture, NIFOS101 and KFRI1212 germinated 94% and 70% of G. elata seeds, respectively, although the initiation of protocorm development was induced much earlier by KFRI 1212 than by NIFOS101.
Furthermore, the NIFOS101 mycelia grew much faster than KFRI 1212 at all temperatures tested. A phylogenetic analysis using the internal transcribed spacer (ITS) sequences showed that NIFOS101 belonged to a clade with M. purpureofusca, which completely differed from the clade with KFRI1212. This study not only identified a new fungal species, NIFOS101, which improved the rate of symbiotic seed germination up to 94% as compared to KFRI1212 (70%), but also revealed that G. elata required a broad taxonomic range of fungi for its symbiotic germination.
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참고문헌 (17)
참고문헌 신청
Bin H / 2004 / Preparative isolation and purification of gastrodin from the chinese medicinal plant Gastrodia elata by high-speed counter current chromatography / J Chromatog
Chang HM / 1986 / Pharmacology and applica-tion of chinese materia medica / World Scientific Publishing, Singapore
Hayashi J / 2002 / Phenolic compounds from Gastrodia rhizoma and relaxant effects of related compounds on isolated smooth muscle preparation / Phytochemistry 59 : 513 ~ 519
Huang ZL / 1985 / Recent developments in pharmacological study and clinical application of Gastrodia elata in china / Chung-His-I-Chieh-Ho-Tsa-Chih 5 : 251 ~ 254
Lee JY / 1982 / The propagation of Gastrodia elata by using rhizomorphs of Armillariella mella / Seoul Women’s University. Rural Development Res Service 7 : 39 ~ 45
Chang HM / 1986 / Pharmacology and applica-tion of chinese materia medica / World Scientific Publishing, Singapore
Hayashi J / 2002 / Phenolic compounds from Gastrodia rhizoma and relaxant effects of related compounds on isolated smooth muscle preparation / Phytochemistry 59 : 513 ~ 519
Huang ZL / 1985 / Recent developments in pharmacological study and clinical application of Gastrodia elata in china / Chung-His-I-Chieh-Ho-Tsa-Chih 5 : 251 ~ 254
Lee JY / 1982 / The propagation of Gastrodia elata by using rhizomorphs of Armillariella mella / Seoul Women’s University. Rural Development Res Service 7 : 39 ~ 45
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