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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 176 - 181 (6page)
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초록· 키워드
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Bcl-x, a member of the Bcl-2 family, plays a key role in apoptosis. Alternative splicing of Bcl-x pre-mRNA through alternative5’ splice-site selection produces an anti-apoptotic mRNAisoform that includes exon 2b and a pro-apoptotic Bcl-x mRNAisoform that excludes exon 2b. Here we used Bcl-x minigeneand identified SRSF2 and SRSF6 as two regulatory factors of 5’splice-site selection of Bcl-x pre-mRNA. We selected bindingclusters closer to 5’ splice-sites from multiple potential bindingsites of SRSF2 and SRSF6 to perform loss of functions analysisthrough site-directed mutagenesis. Our results demonstratedthat these mutations did not abolish regulatory functions ofSRSF2 or SRSF6, indicating that a single binding motif or acluster was not a functional target of these proteins in Bcl-xpre-mRNA splicing. Random deletion mutagenesis did notdisrupt the role of SRSF2 and SRSF6. Importantly, mutagenesisof 5’ splice-site to a conserved or a weaker score demonstratedthat the weaker strength of the target 5’ splice-site or higherstrength of the other 5’ splice-site strength limited the role ofSRSF2 and SRSF6 in 5’ splice-site activation.
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참고문헌 (28)
참고문헌 신청
Wang L / 2016 / Transcriptomic Characterization of SF3B1 Mutation Reveals Its Pleiotropic Effects in Chronic Lymphocytic Leukemia / Cancer Cell 30:750~763
Yin S / 2019 / A Murine Model of Chronic Lymphocytic Leukemia Based on B Cell-Restricted Expression of Sf3b1 Mutation and Atm Deletion / Cancer Cell 35:283e285~296e285
Sumantran VN / 1995 / Overexpression of Bcl-XS sensitizes MCF-7 cells to chemotherapy-induced apoptosis / Cancer Res 55:2507~2510
Mercatante DR / 2001 / Modification of alternative splicing of Bcl-x pre-mRNA in prostate and breast cancer cells. analysis of apoptosis and cell death / J Biol Chem 276:16411~16417
Espana L / 2004 / Overexpression of Bcl-xL in human breast cancer cells enhances organ-selective lymph node metastasis / Breast Cancer Res Treat 87:33~44
Yin S / 2019 / A Murine Model of Chronic Lymphocytic Leukemia Based on B Cell-Restricted Expression of Sf3b1 Mutation and Atm Deletion / Cancer Cell 35:283e285~296e285
Sumantran VN / 1995 / Overexpression of Bcl-XS sensitizes MCF-7 cells to chemotherapy-induced apoptosis / Cancer Res 55:2507~2510
Mercatante DR / 2001 / Modification of alternative splicing of Bcl-x pre-mRNA in prostate and breast cancer cells. analysis of apoptosis and cell death / J Biol Chem 276:16411~16417
Espana L / 2004 / Overexpression of Bcl-xL in human breast cancer cells enhances organ-selective lymph node metastasis / Breast Cancer Res Treat 87:33~44
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