지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 641 - 647 (7page)
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초록· 키워드
오류제보하기
Background/Aims: Helicobacter pylori eradication rates are decreasing because of increases in clarithromycin resistance. Thus, finding an easy and accurate method of detecting clarithromycin resistance is important. Methods: We evaluated 70 H. pylori isolates from Korean patients. Duallabeled peptide nucleic acid (PNA) probes were designed to detect resistance associated with point mutations in 23S ribosomal ribonucleic acid gene domain V (A2142G, A2143G, and T2182C). Data were analyzed by probe-based fluorescence melting curve analysis based on probe-target dissociation temperatures and compared with Sanger sequencing. Results: Among 70 H. pylori isolates, 0, 16, and 58 isolates contained A2142G, A2143G, and T2182C mutations, respectively. PNA probe-based analysis exhibited 100.0% positive predictive values for A2142G and A2143G and a 98.3% positive predictive value for T2182C. PNA probe-based analysis results correlated with 98.6% of Sanger sequencing results (κ-value=0.990; standard error, 0.010). Conclusions: H. pylori clarithromycin resistance can be easily and accurately assessed by dual-labeled PNA probe-based melting curve analysis if probes are used based on the appropriate resistance-related mutations. This method is fast, simple, accurate, and adaptable for clinical samples. It may help clinicians choose a precise eradication regimen.
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참고문헌 (9)
참고문헌 신청
김나영 / 2009 / 헬리코박터 파일로리 감염의 진단 및 치료 가이드라인 / 대한소화기학회지 54(5):269~278
김성은 / 2015 / Trends in Helicobacter pylori eradication rates by first-line triple therapy and related factors in eradication therapy / The Korean Journal of Internal Medicine 30(6):801~807
허준 / 2014 / 한국에서 헬리코박터 파일로리에 대한 삼제요법의 치료율 변화 / 대한소화기학회지 63(3):141~145
송민식 / 2015 / Easy Method for Discriminating the Origins of Manila Clam Ruditapes philippinarum with a Dual-labelled PNA-probe-based Melting Curve Analysis / BioChip Journal 9(3):247~258
김정목 / 2008 / Gene Mutations of 23S rRNA Associated with Clarithromycin Resistance in Helicobacter pylori Strains Isolated from Korean Patients / Journal of Microbiology and Biotechnology 18(9):1584~1589
김성은 / 2015 / Trends in Helicobacter pylori eradication rates by first-line triple therapy and related factors in eradication therapy / The Korean Journal of Internal Medicine 30(6):801~807
허준 / 2014 / 한국에서 헬리코박터 파일로리에 대한 삼제요법의 치료율 변화 / 대한소화기학회지 63(3):141~145
송민식 / 2015 / Easy Method for Discriminating the Origins of Manila Clam Ruditapes philippinarum with a Dual-labelled PNA-probe-based Melting Curve Analysis / BioChip Journal 9(3):247~258
김정목 / 2008 / Gene Mutations of 23S rRNA Associated with Clarithromycin Resistance in Helicobacter pylori Strains Isolated from Korean Patients / Journal of Microbiology and Biotechnology 18(9):1584~1589
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