지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 247 - 258 (12page)
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초록· 키워드
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Probe-based fluorescence melting curveanalysis is a powerful tool used to detect mutations,based on the melting temperature from the thermal denaturation of the probe-target hybrid. PNA (Peptidenucleic acid) probes have several advantages: easy todesign and modify and do not give false negative results. In this study, we developed and applied a MeltingArray method using PNA-probe-based fluorescence melting curve analysis to quickly determine the origins of manila clams. Five PNA probes were designed to discriminate between imported (from Dalian, China) and domestic (Korea) manila clams and positioned at KRph27135, KRph29263 and KRph37987. The KRph 37987-159C PNA probe had the highest discrimination ability. The KRph37987-159C probe had perfect match binding with the domestic manila clams at 80.8-100%, and mismatch binding with the imported manila clams excluding heterozygous samples at 66.7-76.8%. Moreover, domestic manila clams were discriminated by the PNA probes KRph29263-143G and KRph29263-205C at a ratio of 96.8% and 100%, and the imported manila clams were discriminated 100% and 98.5%, respectively. Here, we report that domestic and imported manila clams can be distinguished by the MeltingArray system and that it is a more rapid, simple and accurate method than that of sanger sequencing. The dual-labeled PNA probes offer the advantage of improved flexibility in probe design, which could be used in future applications for genotyping a wide range of subjects.
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참고문헌 (23)
참고문헌 신청
An, H.S. / 2012 / Genetic structure of Korean populations of the clam Ruditapes philippinarum inferred from microsatellite marker analysis. / Biochem. Syst. Ecol 44:186~195
Bernard, P.S. / 1998 / Homogeneous Multiplex Genotyping of Hemochromatosis Mutations with Fluorescent Hybridization Probes / Am. J. Pathol 153:1055~1061
Brandt, O. / 2003 / PNA microarrays for hybridisation of unlabelled DNA samples / Nucleic Acids Research 31:e119
최영진 / 2012 / Evaluation of Peptide Nucleic Acid Probe-based Real-time PCR for Detection of Mycobacterium tuberculosis Complex and Nontuberculous Mycobacteria in Respiratory Specimens / Annals of Laboratory Medicine 32(4):257~263
Choi, Y.J. / 2011 / Evaluation of peptide nucleic acid array for the detection of hepatitis B virus mutations associated with antiviral resistance / Archives of Virology 156:1517~1524
Bernard, P.S. / 1998 / Homogeneous Multiplex Genotyping of Hemochromatosis Mutations with Fluorescent Hybridization Probes / Am. J. Pathol 153:1055~1061
Brandt, O. / 2003 / PNA microarrays for hybridisation of unlabelled DNA samples / Nucleic Acids Research 31:e119
최영진 / 2012 / Evaluation of Peptide Nucleic Acid Probe-based Real-time PCR for Detection of Mycobacterium tuberculosis Complex and Nontuberculous Mycobacteria in Respiratory Specimens / Annals of Laboratory Medicine 32(4):257~263
Choi, Y.J. / 2011 / Evaluation of peptide nucleic acid array for the detection of hepatitis B virus mutations associated with antiviral resistance / Archives of Virology 156:1517~1524
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