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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 107 - 115 (9page)
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초록· 키워드
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Purpose: Glehnia littoralis has been used to treat ischemic stroke, phlegm, cough, systemic paralysis, antipyretics and neuralgia. The pharmacological mechanisms of Glehnia littoralis include calcium channel block, coumarin derivatives, anticoagulation, anti-convulsive effect, as well as anti-oxidant and anti-inflammatory effects. Alpha-amanitin (α-amanitin) is a major toxin from extremely poisonous Amanita fungi. Oxidative stress, which may contribute to severe hepatotoxicity was induced by α-amanitin. The aim of this study was to investigate whether Glehnia littoralis ethyl acetate extract (GLEA) has the protective antioxidant effects on α-amanitin -induced hepatotoxicity.
Methods: Human hepatoma cell line HepG2 cells were pretreated in the presence or absence of GLEA (50, 100 and 200μg/ml) for 4 hours, then exposed to 60μmol/L ofα-amanitin for an additional 4 hours. Cell viability was evaluated using the MTT method. AST, ALT, and LDH production in a culture medium and intracellular MDA, GSH, and SOD levels were determined.
Results: GLEA (50, 100 and 200μg/ml) significantly increased the relative cell viability by 7.11, 9.87, and 14.39%, respectively, and reduced the level of ALT by 10.39%, 34.27%, and 52.14%, AST by 9.89%, 15.16%, and 32.84%, as well as LDH by 15.86%, 22.98%, and 24.32% in culture medium, respectively. GLEA could also remarkably decrease the level of MDA and increase the content of GSH and SOD in the HepG2 cells.
Conclusion: In the in vitro model, Glehnia littoralis was effective in limiting hepatic injury afterα-amanitin poisoning.
Its antioxidant effect is attenuated by antidotal therapy.
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참고문헌 (22)
참고문헌 신청
DUNDAR ZD / 2017 / The role of oxidative stress inα-amanitin-induced hepatotoxicity in an experimental mouse model / Turk J Med Sci 47
Deutsch M / 2007 / Amanita phalloides poisoning: reassessment of prognostic factors and indications for emergency liver transplantation / J hepatol 47 : 728 ~ 729
Enjalbert F / 2002 / Treatment of amatoxin poisoning:20-year retrospective analysis / J Toxicol Clin Toxicol 40 : 715 ~ 757
Fang X / 1986 / The immunosuppressive activities of polysaccharides from Glehnia littoralis (bei sha shen) / Yao xue xue bao 21 : 931 ~ 934
Han M / 1995 / Effects of 24 Chinese medicinal herbs on nucleic acid, protein and cell cycle of human lung adenocarcinoma cell / Zhongguo Zhong Xi Yi Jie He Za Zhi 15 : 147 ~
Deutsch M / 2007 / Amanita phalloides poisoning: reassessment of prognostic factors and indications for emergency liver transplantation / J hepatol 47 : 728 ~ 729
Enjalbert F / 2002 / Treatment of amatoxin poisoning:20-year retrospective analysis / J Toxicol Clin Toxicol 40 : 715 ~ 757
Fang X / 1986 / The immunosuppressive activities of polysaccharides from Glehnia littoralis (bei sha shen) / Yao xue xue bao 21 : 931 ~ 934
Han M / 1995 / Effects of 24 Chinese medicinal herbs on nucleic acid, protein and cell cycle of human lung adenocarcinoma cell / Zhongguo Zhong Xi Yi Jie He Za Zhi 15 : 147 ~
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