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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Water supplied through dental unit waterlines (DUWLs) has been shown to contain high number of bacteria. To reduce the contamination ofDUWLs, it is essential to develop effective disinfectants. It is, however, difficulty to obtain proper DUWL samples for studies. The purpose of thisstudy was to establish a simple laboratory model for reproducing DUWL biofilms. The bacteria obtained from DUWLs were cultured in R2A liquidmedium for 10 days, and then stored at −70oC. This stock was inoculated into R2A liquid medium and incubated in batch mode. After 5 days ofculturing, it was inoculated into the biofilm formation model developed in this study. Our biofilm formation model comprised of a beaker containingR2A liquid medium and five glass rods attached to DUWL polyurethane tubing. Biofilm was allowed to form on the stir plate and the medium wasreplaced every 2 days. After 4 days of biofilm formation in the laboratory model, biofilm thickness, morphological characteristics and distributionof the composing bacteria were examined by confocal laser microscopy and scanning electron microscopy. The mean of biofilm accumulation was4.68×104 colony forming unit/cm2 and its thickness was 10∼14 m. In our laboratory model, thick bacterial lumps were observed in some partsof the tubing. To test the suitability of this biofilm model system, the effectiveness of disinfectants such as sodium hypochlorite, hydrogen peroxide,and chlorhexidine, was examined by their application to the biofilm formed in our model. Lower concentrations of disinfectants were less effectivein reducing the count of bacteria constituting the biofilm. These results showed that our DUWL biofilm laboratory model was appropriate forcomparison of disinfectant effects. Our laboratory model is expected to be useful for various other purposes in further studies.
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참고문헌 (28)
참고문헌 신청
Williams JF / 1993 / Microbial contamination of dental unit waterlines: prevalence, intensity and microbiological characteristics / J Am Dent Assoc 124:59~65
Barbeau J / 1996 / Multiparametric analysis of waterline contamination in dental units / Appl Environ Microbiol 62:3954~3959
Lee BM / 1998 / A study on the microbial contamination of dental unit and ultrasonic scaler / J Korean Acad Prosthodont 36:64~80
Meiller TF / 1999 / Dental unit waterlines: biofilms, disinfection and recurrence / J Am Dent Assoc 130:65~72
Walker JT / 2000 / Microbial biofilm formation and contamination of dental-unit water systems in general dental practice / Appl Environ Microbiol 66:3363~3367
Barbeau J / 1996 / Multiparametric analysis of waterline contamination in dental units / Appl Environ Microbiol 62:3954~3959
Lee BM / 1998 / A study on the microbial contamination of dental unit and ultrasonic scaler / J Korean Acad Prosthodont 36:64~80
Meiller TF / 1999 / Dental unit waterlines: biofilms, disinfection and recurrence / J Am Dent Assoc 130:65~72
Walker JT / 2000 / Microbial biofilm formation and contamination of dental-unit water systems in general dental practice / Appl Environ Microbiol 66:3363~3367
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