지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 충남대학교 농업과학연구소 Korean Journal of Agricultural Science Korean Journal of Agricultural Science Vol.46 No.3
- 발행연도
- 2019.9
- 수록면
- 471 - 488 (18page)
이용수
초록· 키워드
오류제보하기
Random genes were screened in two transforming ways to investigate the new genes of a ladybug using the Harmonia axyridis cDNA library stock cell cloned in the LITMUS 28i vector in a previous study. Phenotypic variation was observed after injection of the synthesized double-stranded RNA through the in vitro transcription process. The cDNA library of H. axyridis was transformed into E. coli DH5α and 10B competent cells by heat shock. Analysis of the nucleotide sequences of the 42 clones with the insert DNAs revealed that 21 clones were homologous with the genes of insects, and only one clone had a gene from H. axyridis. Thirteen of the 21 insect genes were homologous with genes from coleopteran insects. Fourteen genes were selected, which were identified by the gene screening results, and were synthesized as double-stranded RNA through in vitro transcription. One microgram of the synthesized double-stranded RNA between segments T1 and T2 were injected using a syringe into each anesthetized fourth larvae which were under 2 days old. As a result, a phenotypic variation appeared in the larva injected with the two genes. While the eggs of H. axyridis injected with distilled water hatched out three days after oviposition, the eggs of H. axyridis injected with dsHma 06 did not hatch but become shrivel a week after oviposition. Most of the H. axyridis injected with dsHma 08 died and were unable to complete the pupation or eclosion during ecdysis.
목차
Abstract
Introduction
Materials and Methods
Results
Discussion
Conclusion
References
참고문헌 (77)
참고문헌 신청
Amdam GV / 2003 / Disruption of vitellogenin gene function in adult honeybees by intra-abdominal injection of double-stranded RNA / BMC Biotechnology 3:1
Antonio DSM / 2008 / RNAi-mediated silencing of vitellogenin gene function turns honeybee(Apis mellifera)workers into extremely precocious foragers / Naturwissenschaften 95:953~961
Baum JA / 2007 / Control of coleopteran insect pests through RNA interference / Nature Biotechnology 25:1322~1326
Belicek J / 1976 / Coccinellidae of Western Canada and Alaska with analyses of the transmontane zoogeographic relationship between the fauna of British Columbia and Alberta / Questiones Entomologicaes 12:283~409
Bellés X / 2010 / Beyond Drosophila : RNAi In vivo and functional genomics in insects / Annual Review of Entomology 55:111~128
Antonio DSM / 2008 / RNAi-mediated silencing of vitellogenin gene function turns honeybee(Apis mellifera)workers into extremely precocious foragers / Naturwissenschaften 95:953~961
Baum JA / 2007 / Control of coleopteran insect pests through RNA interference / Nature Biotechnology 25:1322~1326
Belicek J / 1976 / Coccinellidae of Western Canada and Alaska with analyses of the transmontane zoogeographic relationship between the fauna of British Columbia and Alberta / Questiones Entomologicaes 12:283~409
Bellés X / 2010 / Beyond Drosophila : RNAi In vivo and functional genomics in insects / Annual Review of Entomology 55:111~128
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