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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.10
- 수록면
- 412 - 418 (7page)
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초록· 키워드
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Background: Ginsenoside Rg3(S) and compound K (C-K) are pharmacologically active components of ginseng that promote human health and improve quality of life. The aim of this study was to produce Rg3(S) and C-K from ginseng extract using recombinant Lactococcus lactis.
Methods: L. lactis subsp. cremoris NZ9000 (L. lactis NZ9000), which harbors β-glucosidase genes (BglPm and BglBX10) from Paenibacillus mucilaginosus and Flavobacterium johnsoniae, respectively, was reacted with ginseng extract (protopanaxadiol-type ginsenoside mixture).
Results: Crude enzyme activity of BglBX10 values comprised 0.001 unit/mL and 0.003 unit/mL in uninduced and induced preparations, respectively. When whole cells of L. lactis harboring pNZBglBX10 were treated with ginseng extract, after permeabilization of cells by xylene, Rb1 and Rd were converted into Rg3(S) with a conversion yield of 61%. C-K was also produced by sequential reactions of the permeabilized cells harboring each pNZBgl and pNZBglBX10, resulting in a 70% maximum conversion yield.
Conclusion: This study demonstrates that the lactic acid bacteria having specific β-glucosidase activity can be used to enhance the health benefits of Panax ginseng in either fermented foods or bioconversion processes.
Methods: L. lactis subsp. cremoris NZ9000 (L. lactis NZ9000), which harbors β-glucosidase genes (BglPm and BglBX10) from Paenibacillus mucilaginosus and Flavobacterium johnsoniae, respectively, was reacted with ginseng extract (protopanaxadiol-type ginsenoside mixture).
Results: Crude enzyme activity of BglBX10 values comprised 0.001 unit/mL and 0.003 unit/mL in uninduced and induced preparations, respectively. When whole cells of L. lactis harboring pNZBglBX10 were treated with ginseng extract, after permeabilization of cells by xylene, Rb1 and Rd were converted into Rg3(S) with a conversion yield of 61%. C-K was also produced by sequential reactions of the permeabilized cells harboring each pNZBgl and pNZBglBX10, resulting in a 70% maximum conversion yield.
Conclusion: This study demonstrates that the lactic acid bacteria having specific β-glucosidase activity can be used to enhance the health benefits of Panax ginseng in either fermented foods or bioconversion processes.
목차
abstract
1. Introduction
2. Materials and methods
3. Results and discussion
4. Conclusions
References
참고문헌 (26)
참고문헌 신청
[학술지(정기간행물)] - Chang KH / 2009 / Optimization of the enzymatic production of 20(S)-ginsenoside Rg3 from white ginseng extract using response surface methodology / New Biotechnol 26 : 181 ~ 186
[학술지(정기간행물)] - Park CS / 2010 / Biotransformation of ginsenosides by hydrolyzing the sugar moieties of ginsenosides using microbial glycosidases / Appl Microbiol Biotechnol 87 : 9 ~ 19
[학술지(정기간행물)] - 백인호 / 2013 / The world ginseng market and the ginseng (Korea) / Journal of Ginseng Research 37 (1) : 1 ~ 7
[학술지(정기간행물)] - Myung Kyum Kim / 2005 / Microbial Conversion of Major Ginsenoside Rb1 to Pharmaceutically Active Minor Ginsenoside Rd / The Journal of Microbiology 43 (5) : 456 ~ 462
[학술지(정기간행물)] - Jitendra Upadhyaya / 2016 / Enzymatic formation of compound-K from ginsenoside Rb1 by enzyme preparation from cultured mycelia of Armillaria mellea / Journal of Ginseng Research 40 (2) : 105 ~ 112
[학술지(정기간행물)] - Park CS / 2010 / Biotransformation of ginsenosides by hydrolyzing the sugar moieties of ginsenosides using microbial glycosidases / Appl Microbiol Biotechnol 87 : 9 ~ 19
[학술지(정기간행물)] - 백인호 / 2013 / The world ginseng market and the ginseng (Korea) / Journal of Ginseng Research 37 (1) : 1 ~ 7
[학술지(정기간행물)] - Myung Kyum Kim / 2005 / Microbial Conversion of Major Ginsenoside Rb1 to Pharmaceutically Active Minor Ginsenoside Rd / The Journal of Microbiology 43 (5) : 456 ~ 462
[학술지(정기간행물)] - Jitendra Upadhyaya / 2016 / Enzymatic formation of compound-K from ginsenoside Rb1 by enzyme preparation from cultured mycelia of Armillaria mellea / Journal of Ginseng Research 40 (2) : 105 ~ 112
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