Distribution Status of Hybrid Types in Large Liver Flukes, Fasciola Species (Digenea: Fasciolidae), from Ruminants and Humans in Vietnam

Thi Bich Nga Nguyen; Nguyen Van De; Thi Kim Lan Nguyen; Huynh Hong Quang; Huong Thi Thanh Doan; Takeshi Agatsuma; Thanh Hoa Le

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저자정보: Thi Bich Nga Nguyen (Vietnam Academy of Science and Technology (VAST)) Nguyen Van De (Hanoi Medical University) Thi Kim Lan Nguyen (Thai Nguyen University) Huynh Hong Quang (Nguyen Thai Hoc) Huong Thi Thanh Doan (Vietnam Academy of Science and Technology (VAST)) Takeshi Agatsuma (Kochi University) Thanh Hoa Le (Vietnam Academy of Science and Technology (VAST))

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.56 No.5

발행연도: 2018.10

수록면: 453 - 461 (9page)

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The aim of this study is to delineate ‘admixed hybrid’ and ‘introgressive’ Fasciola genotypes present in the Fasciola population in Vietnam. Adult liver flukes collected from ruminants in 18 Provinces were morphologically sorted out by naked eyes for small (S), medium (M) and large (L) body shapes; and human samples (n=14) from patients. Nuclear ribosomal (rDNA) ITS1 and ITS2, and mitochondrial (mtDNA) nad1 markers were used for determination of their genetic status. Total 4,725 worm samples of ruminants were tentatively classified by their size: 6% (n=284) small (S)-, 13% (n=614) medium (M)-, and 81% (n=3,827) large (L)-forms. All the representative (n=120, as 40 each group) and 14 human specimens, possessed maternal mtDNA of only F. gigantica and none of F. hepatica. Paternally, all (100%) of the L- (n=40) and 77.5% (n=31) of the M-flukes had single F. gigantica rDNA indicating ‘pure’ F. gigantica. A majority (90%, n=36) of the S- and 15% (n=6) of the M-worms had single F. hepatica rDNA, indicating their introgressive; the rest (10%, n=4) of the S- and 7.5% (n=3) of the M-flukes had mixture of both F. gigantica and F. hepatica rDNAs, confirming their admixed hybrid genetic status. Fourteen human samples revealed 9 (64%) of pure F. gigantica, 3 (22%) of introgressive and 2 (14%) of admixed hybrid Fasciola spp. By the present study, it was confirmed that the small worms, which are morphologically identical with F. hepatica, are admixed and/or introgressive hybrids of Fasciola spp., and able to be the pathogens of human fascioliasis.

#Fasciola gigantica #F. hepatica #admixed hybrid/introgressive Fasciola spp. #Vietnam

참고문헌 (39)

참고문헌 신청

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