지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한외과학회 Annals of Surgical Treatment and Research Annals of Surgical Treatment and Research Vol.95 No.2
- 발행연도
- 2018.8
- 수록면
- 55 - 63 (9page)
이용수
초록· 키워드
오류제보하기
Purpose: To determine the occurrence of COX-2 methylation in gastric carcinoma (GC), the status and level of CpG methylation in the promoter region of cyclooxygenase-2 (COX-2) were analyzed in early and advanced GCs, as well as in normal gastric tissues.
Methods: The extent of promoter methylation of the COX-2 gene was assessed quantitatively using pyrosequencing in 60 early and 60 advanced GCs samples harvested upon gastrectomy, and 40 normal gastric mucosa samples from patients with benign gastric diseases as controls.
Results: The methylation frequency for the COX-2 gene was significantly higher in early than in advanced GCs (40.0% vs. 20.0%, P < 0.05). A significant difference was found in COX-2 methylation between GCs and normal gastric tissues (30.0% vs. 10.0%, by PS; P < 0.05). COX-2 gene methylation was significantly associated with the depth of invasion (P = 0.003), lymph node metastasis (P = 0.009), distant metastasis (P = 0.036), and TNM staging (P = 0.007). The overall survival of patients with COX-2 methylation was significantly lower than that of patients without COX-2 methylation (P = 0.005).
Conclusion: These results demonstrated that COX-2 promoter methylation was significantly higher in tumor tissues, and was an early event for GC, thus, COX-2 gene methylation may be important in the initial development of gastric carcinogenesis. Thus, GCs with methylation in COX-2 may not be good candidates for treatment with COX-2 inhibitors. Furthermore, COX-2 methylation could be a significant prognostic factor predicting a favorable effect on GC patient outcome when downregulated.
Methods: The extent of promoter methylation of the COX-2 gene was assessed quantitatively using pyrosequencing in 60 early and 60 advanced GCs samples harvested upon gastrectomy, and 40 normal gastric mucosa samples from patients with benign gastric diseases as controls.
Results: The methylation frequency for the COX-2 gene was significantly higher in early than in advanced GCs (40.0% vs. 20.0%, P < 0.05). A significant difference was found in COX-2 methylation between GCs and normal gastric tissues (30.0% vs. 10.0%, by PS; P < 0.05). COX-2 gene methylation was significantly associated with the depth of invasion (P = 0.003), lymph node metastasis (P = 0.009), distant metastasis (P = 0.036), and TNM staging (P = 0.007). The overall survival of patients with COX-2 methylation was significantly lower than that of patients without COX-2 methylation (P = 0.005).
Conclusion: These results demonstrated that COX-2 promoter methylation was significantly higher in tumor tissues, and was an early event for GC, thus, COX-2 gene methylation may be important in the initial development of gastric carcinogenesis. Thus, GCs with methylation in COX-2 may not be good candidates for treatment with COX-2 inhibitors. Furthermore, COX-2 methylation could be a significant prognostic factor predicting a favorable effect on GC patient outcome when downregulated.
목차
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (29)
참고문헌 신청
[학술지(정기간행물)] - Toyota M / 1999 / Aberrant methylation in gastric cancer associated with the CpG island methylator phenotype / Cancer Res 59 : 5438 ~ 5442
[학술지(정기간행물)] - Momparler RL / 2000 / DNA methylation and cancer / J Cell Physiol 183 : 145 ~ 154
[학술지(정기간행물)] - Barlow DP / 1995 / Gametic imprinting in mammals / Science 270 : 1610 ~ 1613
[학술지(정기간행물)] - O'Neill RJ / 1998 / Undermethylation associated with retroelement activation and chromosome remodelling in an interspecific mammalian hybrid / Nature 393 : 68 ~ 72
[학술지(정기간행물)] - Baylin SB / 1998 / Alterations in DNA methylation: a fundamental aspect of neoplasia / Adv Cancer Res 72 : 141 ~ 196
[학술지(정기간행물)] - Momparler RL / 2000 / DNA methylation and cancer / J Cell Physiol 183 : 145 ~ 154
[학술지(정기간행물)] - Barlow DP / 1995 / Gametic imprinting in mammals / Science 270 : 1610 ~ 1613
[학술지(정기간행물)] - O'Neill RJ / 1998 / Undermethylation associated with retroelement activation and chromosome remodelling in an interspecific mammalian hybrid / Nature 393 : 68 ~ 72
[학술지(정기간행물)] - Baylin SB / 1998 / Alterations in DNA methylation: a fundamental aspect of neoplasia / Adv Cancer Res 72 : 141 ~ 196
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UCI(KEPA) : I410-ECN-0101-2018-514-003356196