지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.7
- 수록면
- 542 - 549 (8page)
이용수
초록· 키워드
오류제보하기
In this study, a peptide exhibiting antioxidant activity was isolated from sandfish (Arctoscopus japonicus) roe hydrolysate (SRH) in order to evaluate their practical uses as materials for manufacturing functional foods. The A. japonicus roe protein was hydrolyzed using Collupulin MG, and isolation of antioxidant peptide was performed using ultrafiltration (UF), prep-HPLC, and RP-HPLC. The SRH with a molecular weight below 3 kDa constituted about 38% of the whole hydrolysate, and the fraction with a molecular weight below 3 kDa showed significantly greater antioxidant activity compared to the original SRH and other fractions. The isolation fold of the antioxidant peptide isolated from SRH throughout the four-step procedure was 7.11-fold, and protein yield was 14.8%. The DPPH radical scavenging activity of isolated antioxidant peptide was above 90% at a concentration of 1.0 mg/mL, which was similar to that of the Trolox at a concentration of 0.1 mg/mL. These results suggested that the antioxidant peptide derived from A. japonicus roe could be a useful additive for producing functional foods and protein supplements. However, it is necessary to perform further study the structural characteristics of this antioxidant peptide isolated from A. japonicus roe.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
References
참고문헌 (36)
참고문헌 신청
[학술지(정기간행물)] - Lee JK / 2011 / Effect of angiotensin I converting enzyme inhibitory peptide purified from skate skin hydrolysate / Food Chem 125 : 495 ~ 499
[학술지(정기간행물)] - Sheih IC / 2010 / Anticancer and antioxidant activities of the peptide fraction from algae protein waste / J Agric Food Chem 58 : 1202 ~ 1207
[학술지(정기간행물)] - Liu Z / 2008 / Production of cysteine-rich antimicrobial peptide by digestion of oyster(Crassostrea gigas)with alcalase and bromelin / Food Control 19 : 231 ~ 235
[학술지(정기간행물)] - Mendis E / 2005 / Antioxidant properties of a radical-scavenging peptide purified from enzymatically prepared fish skin gelatin hydrolysate / J Agric Food Chem 53 : 581 ~ 587
[학술지(정기간행물)] - Duarte J / 2006 / Immunomodulating capacity of commercial fish protein hydrolysate for diet supplementation / Immunobiology 211 : 341 ~ 350
[학술지(정기간행물)] - Sheih IC / 2010 / Anticancer and antioxidant activities of the peptide fraction from algae protein waste / J Agric Food Chem 58 : 1202 ~ 1207
[학술지(정기간행물)] - Liu Z / 2008 / Production of cysteine-rich antimicrobial peptide by digestion of oyster(Crassostrea gigas)with alcalase and bromelin / Food Control 19 : 231 ~ 235
[학술지(정기간행물)] - Mendis E / 2005 / Antioxidant properties of a radical-scavenging peptide purified from enzymatically prepared fish skin gelatin hydrolysate / J Agric Food Chem 53 : 581 ~ 587
[학술지(정기간행물)] - Duarte J / 2006 / Immunomodulating capacity of commercial fish protein hydrolysate for diet supplementation / Immunobiology 211 : 341 ~ 350
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UCI(KEPA) : I410-ECN-0101-2018-059-001945362