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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.10
- 수록면
- 314 - 321 (8page)
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초록· 키워드
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Background: Ginseng belongs to the genus Panax. Its main active ingredients are the ginsenosides. Interstitial cells of Cajal (ICCs) are the pacemaker cells of the gastrointestinal (GI) tract. To understand the effects of ginsenoside Re (GRe) on GI motility, the authors investigated its effects on the pacemaker activity of ICCs of the murine small intestine.
Methods: Interstitial cells of Cajal were dissociated from mouse small intestines by enzymatic digestion. The whole-cell patch clamp configuration was used to record pacemaker potentials in cultured ICCs. Changes in cyclic guanosine monophosphate (cGMP) content induced by GRe were investigated.
Results: Ginsenoside Re (20-40μM) decreased the amplitude and frequency of ICC pacemaker activity in a concentration-dependent manner. This action was blocked by guanosine 50-[b-thio]diphosphate [a guanosine-5’-triphosphate (GTP)-binding protein inhibitor] and by glibenclamide [an adenosine triphosphate (ATP)-sensitive Kþ channel blocker]. To study the GRe-induced signaling pathway in ICCs, the effects of 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (a guanylate cyclase inhibitor) and RP-8-CPTcGMPS (a protein kinase G inhibitor) were examined. Both inhibitors blocked the inhibitory effect of GRe on ICC pacemaker activity. L-NG-nitroarginine methyl ester (100μM), which is a nonselective nitric oxide synthase (NOS) inhibitor, blocked the effects of GRe on ICC pacemaker activity and GRe-stimulated cGMP production in ICCs.
Conclusion: In cultured murine ICCs, GRe inhibits the pacemaker activity of ICCs via the ATP-sensitive potassium (K⁺) channel and the cGMP/NO-dependent pathway. Ginsenoside Re may be a basis for developing novel spasmolytic agents to prevent or alleviate GI motility dysfunction.
Methods: Interstitial cells of Cajal were dissociated from mouse small intestines by enzymatic digestion. The whole-cell patch clamp configuration was used to record pacemaker potentials in cultured ICCs. Changes in cyclic guanosine monophosphate (cGMP) content induced by GRe were investigated.
Results: Ginsenoside Re (20-40μM) decreased the amplitude and frequency of ICC pacemaker activity in a concentration-dependent manner. This action was blocked by guanosine 50-[b-thio]diphosphate [a guanosine-5’-triphosphate (GTP)-binding protein inhibitor] and by glibenclamide [an adenosine triphosphate (ATP)-sensitive Kþ channel blocker]. To study the GRe-induced signaling pathway in ICCs, the effects of 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (a guanylate cyclase inhibitor) and RP-8-CPTcGMPS (a protein kinase G inhibitor) were examined. Both inhibitors blocked the inhibitory effect of GRe on ICC pacemaker activity. L-NG-nitroarginine methyl ester (100μM), which is a nonselective nitric oxide synthase (NOS) inhibitor, blocked the effects of GRe on ICC pacemaker activity and GRe-stimulated cGMP production in ICCs.
Conclusion: In cultured murine ICCs, GRe inhibits the pacemaker activity of ICCs via the ATP-sensitive potassium (K⁺) channel and the cGMP/NO-dependent pathway. Ginsenoside Re may be a basis for developing novel spasmolytic agents to prevent or alleviate GI motility dysfunction.
목차
abstract
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (41)
참고문헌 신청
[학술지(정기간행물)] - Nah SY / 1997 / Ginseng: recent advances and trends / Korean J Ginseng Sci 21 : 1 ~ 12
[학술지(정기간행물)] - Kim HS / 2007 / Effects of ginseng total saponins on pacemaker currents of interstitial cells of Cajal from the small intestine of mice / Biol Pharm Bull 30 : 2037 ~ 2042
[학술지(정기간행물)] - Seungheon Han / 2012 / Effects of Ginsenoside on Pacemaker Potentials of Cultured Interstitial Cells of Cajal Clusters from the Small Intestine of Mice / Molecules and Cells 33 (3) : 243 ~ 249
[학술지(정기간행물)] - Saito H / 1977 / Effects of Panax ginseng root on conditioned avoidance response in rats / Jpn J Pharmacol 27 : 509 ~ 516
[학술지(정기간행물)] - 이창호 / 2014 / A review on the medicinal potentials of ginseng and ginsenosides on cardiovascular diseases / Journal of Ginseng Research 38 (3) : 161 ~ 166
[학술지(정기간행물)] - Kim HS / 2007 / Effects of ginseng total saponins on pacemaker currents of interstitial cells of Cajal from the small intestine of mice / Biol Pharm Bull 30 : 2037 ~ 2042
[학술지(정기간행물)] - Seungheon Han / 2012 / Effects of Ginsenoside on Pacemaker Potentials of Cultured Interstitial Cells of Cajal Clusters from the Small Intestine of Mice / Molecules and Cells 33 (3) : 243 ~ 249
[학술지(정기간행물)] - Saito H / 1977 / Effects of Panax ginseng root on conditioned avoidance response in rats / Jpn J Pharmacol 27 : 509 ~ 516
[학술지(정기간행물)] - 이창호 / 2014 / A review on the medicinal potentials of ginseng and ginsenosides on cardiovascular diseases / Journal of Ginseng Research 38 (3) : 161 ~ 166
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UCI(KEPA) : I410-ECN-0101-2018-524-001597893