Comparison of Placenta PCR and Maternal Serology of Aborted Women for Detection of Toxoplasma gondii in Ardabil, Iran

Somaie Matin; Gholamreza Shahbazi; Shervin Tabrizian Namin; Rouhallah Moradpour; Farideh Feizi; Hadi Piri-dogahe

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저자정보: Somaie Matin (Ardabil University of Medical Sciences) Gholamreza Shahbazi (Islamic Azad University) Shervin Tabrizian Namin (Ardabil University of Medical Sciences) Rouhallah Moradpour (Ardabil University of Medical Sciences) Farideh Feizi (Ardabil University of Medical Sciences) Hadi Piri-dogahe (Ardabil University of Medical Sciences)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.55 No.6

발행연도: 2017.12

수록면: 607 - 611 (5page)

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Primary maternal infection with toxoplasmosis during pregnancy is frequently associated with transplacental transmission of the parasite to the fetus. This study was conducted to test the utility of PCR assay to detect recent infections with Toxoplasma in aborted women at various gestational ages who referred to Obstetrics and Gynecology Department of Alavi Hospital in Ardabil during 2014 and 2016. Two hundred women with a history of single or repeated abortion were investigated in this study. Blood samples were tested for specific anti-Toxoplasma IgM and IgG antibodies by ELISA. According to the results, 53.5% of the women under study were positive for anti-Toxoplasma antibodies: 4.0% of them had IgM, 43.0% had IgG, and 6.5% had both IgM and IgG. Subsequently, Nested-PCR analysis was used to detect T. gondii DNA in the placenta of subjects. In 10.5% of the women, the results were positive for 529 bp element of T. gondii. Among them, 5 (23.8%) cases were IgM positive, 1 (4.8%) case was IgG positive, and 11 (52.4%) were both IgM and IgG positive. In 4 (19.0%) patients, none of the antibodies were found to be positive. In total, 16 patients had positive results in both ELISA and PCR methods, and 174 cases had negative results for new infection. The findings of this study revealed that T. gondii might be one of the significant factors leading to abortion, and that the analysis of placenta can be important in order to achieve increased detection sensitivity.

#Toxoplasma gondii #abortion #blood #nested-PCR #ELISA #Iran

참고문헌 (24)

참고문헌 신청

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[단행본] - Boothroyd JC / 2009 / Encyclopedia of Microbiology / Elsevier : 732 ~ 743

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[학술지(정기간행물)] - McAuley J / 1994 / Early and longitudinal evaluations of treated infants and children and untreated historical patients with congenital toxoplasmosis : the Chicago collaborative treatment trial / Clin Infect Dis 18 : 38 ~ 72

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