지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.10
- 수록면
- 469 - 476 (8page)
이용수
초록· 키워드
오류제보하기
Background: Panax ginseng Meyer (Asian ginseng) has a large nuclear genome size of > 3.5 Gbp in haploid genome equivalent of 24 chromosomes. Tandem repeats (TRs) occupy significant portions of the genome in many plants and are often found in specific genomic loci, making them a valuable molecular cytogenetic tool in discriminating chromosomes. In an effort to understand the P. ginseng genome structure, we characterized an ultrahigh copy 167-bp TR (Pg167TR) and explored its chromosomal distribution as well as its utility for chromosome identification.
Methods: Polymerase chain reaction amplicons of Pg167TR were labeled, along with 5S and 45S rDNA amplicons, using a direct nick-translation method. Direct fluorescence in situ hybridization (FISH) was used to analyze the chromosomal distribution of Pg167TR.
Results: Recently, we reported a method of karyotyping the 24 chromosome pairs of P. ginseng using rDNA and DAPI (40,6-diamidino-2-phenylindole) bands. Here, a unique distribution of Pg167TR in all 24P. ginseng chromosomes was observed, allowing easy identification of individual homologous chromosomes. Additionally, direct labeling of 5S and 45S rDNA probes allowed the identification of two additional 5S rDNA loci not previously reported, enabling the refinement of the P. ginseng karyotype.
Conclusion: Identification of individual P. ginseng chromosomes was achieved using Pg167TR-FISH. Chromosome identification is important in understanding the P. ginseng genome structure, and our method will be useful for future integration of genetic linkage maps and genome scaffold anchoring. Additionally, it is a good tool for comparative studies with related species in efforts to understand the evolution of P. ginseng.
Methods: Polymerase chain reaction amplicons of Pg167TR were labeled, along with 5S and 45S rDNA amplicons, using a direct nick-translation method. Direct fluorescence in situ hybridization (FISH) was used to analyze the chromosomal distribution of Pg167TR.
Results: Recently, we reported a method of karyotyping the 24 chromosome pairs of P. ginseng using rDNA and DAPI (40,6-diamidino-2-phenylindole) bands. Here, a unique distribution of Pg167TR in all 24P. ginseng chromosomes was observed, allowing easy identification of individual homologous chromosomes. Additionally, direct labeling of 5S and 45S rDNA probes allowed the identification of two additional 5S rDNA loci not previously reported, enabling the refinement of the P. ginseng karyotype.
Conclusion: Identification of individual P. ginseng chromosomes was achieved using Pg167TR-FISH. Chromosome identification is important in understanding the P. ginseng genome structure, and our method will be useful for future integration of genetic linkage maps and genome scaffold anchoring. Additionally, it is a good tool for comparative studies with related species in efforts to understand the evolution of P. ginseng.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
References
참고문헌 (38)
참고문헌 신청
[학술지(정기간행물)] - Michael TP / 2013 / The first 50 plant genomes / Plant Genom 6 : 1 ~ 7
[학술지(정기간행물)] - Macas J / 2009 / Hypervariable 30 UTR region of plant LTR-retrotransposons as a source of novel satellite repeats / Gene 448 : 198 ~ 206
[학술지(정기간행물)] - Choi HI / 2014 / Major repeat components covering one-third of the ginseng (Panax ginseng C.A. Meyer) genome and evidence for allotetraploidy / Plant J 77 : 906 ~ 916
[학술지(정기간행물)] - Coluccia E / 2011 / Identification of two new repetitive elements and chromosomal mapping of repetitive DNA sequences in the fish Gymnothorax unicolor (Anguilliformes: Muraenidae) / Eur J Histochem 55 : e12 ~
[학술지(정기간행물)] - Sharma A / 2013 / Tandem repeats derived from centromeric retrotransposons / BMC Genomics 14 : 142 ~
[학술지(정기간행물)] - Macas J / 2009 / Hypervariable 30 UTR region of plant LTR-retrotransposons as a source of novel satellite repeats / Gene 448 : 198 ~ 206
[학술지(정기간행물)] - Choi HI / 2014 / Major repeat components covering one-third of the ginseng (Panax ginseng C.A. Meyer) genome and evidence for allotetraploidy / Plant J 77 : 906 ~ 916
[학술지(정기간행물)] - Coluccia E / 2011 / Identification of two new repetitive elements and chromosomal mapping of repetitive DNA sequences in the fish Gymnothorax unicolor (Anguilliformes: Muraenidae) / Eur J Histochem 55 : e12 ~
[학술지(정기간행물)] - Sharma A / 2013 / Tandem repeats derived from centromeric retrotransposons / BMC Genomics 14 : 142 ~
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UCI(KEPA) : I410-ECN-0101-2018-524-001599226