Molecular Phylogenetics of Trichostrongylus Species (Nematoda: Trichostrongylidae) from Humans of Mazandaran Province, Iran

Meysam Sharifdini; Zahra Heidari; Zahra Hesari; Sajad Vatandoost; Eshrat Beigom Kia

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자료유형: 학술저널

저자정보: Meysam Sharifdini (Guilan University of Medical Sciences) Zahra Heidari (Ardabil University of Medical Sciences) Zahra Hesari (Guilan University of Medical Sciences) Sajad Vatandoost (Tehran University) Eshrat Beigom Kia (Tehran University of Medical Sciences)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.55 No.3

발행연도: 2017.6

수록면: 279 - 285 (7page)

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The present study was performed to analyze molecularly the phylogenetic positions of human-infecting Trichostrongylus species in Mazandaran Province, Iran, which is an endemic area for trichostrongyliasis. DNA from 7 Trichostrongylus infected stool samples were extracted by using in-house (IH) method. PCR amplification of ITS2-rDNA region was performed, and products were sequenced. Phylogenetic analysis of the nucleotide sequence data was performed using MEGA 5.0 software. Six out of 7 isolates had high similarity with Trichostrongylus colubriformis, while the other one showed high homology with Trichostrongylus axei registered in GenBank reference sequences. Intra-specific variations within isolates of T. colubriformis and T. axei amounted to 0-1.8% and 0-0.6%, respectively. Trichostrongylus species obtained in the present study were in a cluster with the relevant reference sequences from previous studies. BLAST analysis indicated that there was 100% homology among all 6 ITS2 sequences of T. colubriformis in the present study and most previously registered sequences of T. colubriformis from human, sheep, and goat isolates from Iran and also human isolates from Laos, Thailand, and France. The ITS2 sequence of T. axei exhibited 99.4% homology with the human isolate of T. axei from Thailand, sheep isolates from New Zealand and Iran, and cattle isolate from USA.

#Trichostrongylus colubriformis #Trichostrongylus axei #human #PCR #ITS2-rDNA region #phylogenetic analysis #Mazandaran #Iran

참고문헌 (34)

참고문헌 신청

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[단행본] - John D / 2006 / Markell and Voge’s Medical Parasitology / Elsevier : 266 ~

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