Potential Vaccine Targets against Rabbit Coccidiosis by Immunoproteomic Analysis

Hongyan Song; Ronglian Dong; Baofeng Qiu; Jin Jing; Shunxing Zhu; Chun Liu; Yingmei Jiang; Shengcun Wang; Jin Miao; Yixiang Shao

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자료유형: 학술저널

저자정보: Hongyan Song (Nantong University) Ronglian Dong (Jiangsu Provincial Center for Disease Control and Prevention) Baofeng Qiu (Nantong Entry-Exit Inspection and Quarantine Bureau) Jin Jing (Nantong University) Shunxing Zhu (Nantong University) Chun Liu (Nantong University) Yingmei Jiang (Nantong University) Shengcun Wang (Nantong University) Jin Miao (Nantong University) Yixiang Shao (Nantong University)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.55 No.1

발행연도: 2017.2

수록면: 15 - 20 (6page)

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The aim of this study was to identify antigens for a vaccine or drug target to control rabbit coccidiosis. A combination of 2-dimensional electrophoresis, immunoblotting, and mass spectrometric analysis were used to identify novel antigens from the sporozoites of Eimeria stiedae. Protein spots were recognized by the sera of New Zealand rabbits infected artificially with E. stiedae. The proteins were characterized by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF/TOF-MS) analysis in combination with bioinformatics. Approximately 868 protein spots were detected by silver-staining, and a total of 41 immunoreactive protein spots were recognized by anti-E. stiedae sera. Finally, 23 protein spots were successfully identified. The proteins such as heat shock protein 70 and aspartyl protease may have potential as immunodiagnostic or vaccine antigens. The immunoreactive proteins were found to possess a wide range of biological functions. This study is the first to report the proteins recognized by sera of infected rabbits with E. stiedae, which might be helpful in identifying potential targets for vaccine development to control rabbit coccidiosis.

#Eimeria stiedae #rabbit #sporozoite #immunoproteomics #vaccine target

참고문헌 (36)

참고문헌 신청

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[학술지(정기간행물)] - Abu-Akkada SS / 2010 / Garlic and hepatic coccidiosis: prophylaxis or treatment? / Trop Anim Health Prod 42 : 1337 ~ 1343

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[학술지(정기간행물)] - Peeters JE / 1986 / Efficacy of toltrazuril against intestinal and hepatic coccidiosis in rabbits / Vet Parasitol 22 : 21 ~ 35

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