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논문 기본 정보

자료유형
학술저널
저자정보
Jung-Min Park (Dongguk University) Kyung-Hwa Chang (Dongguk University) Kwang-Hoon Park (Dongguk University) Seong-Jin Choi (Korea Institute of Toxicology) Kyuhong Lee (Korea Institute of Toxicology) Jin-Yong Lee (Aichi Gakuin University) Masahiko Satoh (Aichi Gakuin University) Seong-Yu Song (Korea University) Moo-Yeol Lee (Dongguk University)
저널정보
한국독성학회 Toxicological Research Toxicological Research Vol.32 No.4
발행연도
2016.10
수록면
353 - 358 (6page)

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초록· 키워드

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The generation and collection of cigarette smoke (CS) is a prerequisite for any toxicology study on smoking, especially an in vitro CS exposure study. In this study, the effects on blood and vascular function were tested with two widely used CS preparations to compare the biological effects of CS with respect to the CS preparation used. CS was prepared in the form of total particulate matter (TPM), which is CS trapped in a Cambridge filter pad, and cigarette smoke extract (CSE), which is CS trapped in phosphate-buffered saline. TPM potentiated platelet reactivity to thrombin and thus increased aggregation at a concentration of 25~100 μg/mL, whereas 2.5~10% CSE decreased platelet aggregation by thrombin. Both TPM and CSE inhibited vascular contraction by phenylephrine at 50~100 μg/mL and 10%, respectively. TPM inhibited acetylcholine-induced vasorelaxation at 10~100 μg/mL, but CSE exhibited a minimal effect on relaxation at the concentration that affects vasoconstriction. Neither TPM nor CSE induced hemolysis of erythrocytes or influenced plasma coagulation, as assessed by prothrombin time (PT) and activated partial thromboplastin time (aPTT). Taken together, CS affects platelet activity and deteriorates vasomotor functions in vitro. However, the effect on blood and blood vessels may vary depending on the CS preparation. Therefore, the results of experiments conducted with CS preparations should be interpreted with caution.

목차

INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

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