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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.12
- 수록면
- 291 - 295 (5page)
이용수
초록· 키워드
오류제보하기
The limited productivity of natural shell matrix proteins has hampered the investigation of their biochemical properties and practical applications, although biominerals in nature obtained by organic-inorganic assemblies have attractive mechanical and biological properties. Here, we prepared a vector for the expression of a fusion protein of a shell matrix protein from Pinctada fucata (named as GRP_BA) with the GRGDSP residue. The fusion protein of BA-RGD was simply produced in E. coli and purified through sequential steps including the treatment with CaCl₂ and EDTA solution for cell membrane washing, mechanical cell disruption and the application of non-ionic surfactant of Triton X-100 for BA-RGD inclusion body washing. The production yield was approximately 60 mg/L, any other protein band was not observed in SDS-PAGE and it was estimated that above 97% endotoxin was removed compared to the endotoxin level of whole cell. This study showed this simple and easy purification approach could be applied to the purification of BA-RGD fusion protein. It is expected that the protein could be utilized for the preparation of biominerals in practical aspects.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHOD
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (25)
참고문헌 신청
[학술지(정기간행물)] - Dhami, N. K. / 2013 / Biomineralization of calcium carbonates and their engineered applications: a review / Front. Microbiol 4 : 31 ~
[단행본] - Feng, Q. / 2011 / Molecular Biomineralization:Aquatic Organisms Forming Extraordinary Materials / Springer-Verlag Berlin Heidelberg : 113 ~ 140
[학술지(정기간행물)] - Wang, X. H / 2014 / Enzymebased biosilica and biocalcite: biomaterials for the future in regenerative medicine / Trends Biotechnol 32 : 441 ~ 447
[학술지(정기간행물)] - Bahn, S. Y. / 2015 / Role of Pif97 in nacre biomineralization: In vitro characterization of recombinant Pif97 as a framework protein for the association of organic-inorganic layers in nacre / Cryst. Growth Des 15 : 3666 ~ 3673
[학술지(정기간행물)] - Belcher, A. M. / 1996 / Control of crystal phase switching and orientation by soluble mollusc-shell proteins / Nature 381 : 56 ~ 58
[단행본] - Feng, Q. / 2011 / Molecular Biomineralization:Aquatic Organisms Forming Extraordinary Materials / Springer-Verlag Berlin Heidelberg : 113 ~ 140
[학술지(정기간행물)] - Wang, X. H / 2014 / Enzymebased biosilica and biocalcite: biomaterials for the future in regenerative medicine / Trends Biotechnol 32 : 441 ~ 447
[학술지(정기간행물)] - Bahn, S. Y. / 2015 / Role of Pif97 in nacre biomineralization: In vitro characterization of recombinant Pif97 as a framework protein for the association of organic-inorganic layers in nacre / Cryst. Growth Des 15 : 3666 ~ 3673
[학술지(정기간행물)] - Belcher, A. M. / 1996 / Control of crystal phase switching and orientation by soluble mollusc-shell proteins / Nature 381 : 56 ~ 58
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UCI(KEPA) : I410-ECN-0101-2016-470-002231726