지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.12
- 수록면
- 296 - 301 (6page)
이용수
초록· 키워드
오류제보하기
Eggshell-based biocomposites have become attractive due to their exquisite nanostructure and biological properties, which are mainly composed of highly organized calcium carbonate crystals controlled by organic macromolecules such as proteins and polysaccharides. Here, we designed the recombinant fusion protein of a putative eggshell matrix protein named as GG1234 and a fluorescent reporter protein of DsRed. The protein was successfully over-expressed in E. coli and purified by Ni-NTA affinity chromatography. In vitro calcium carbonate crystallization was conducted in the presence of the fusion protein, and morphological change was investigated. The protein inhibited the calcite growth in vitro, and spherical calcium carbonate micro-particles with the diameter of about 20-30 μm were obtained. We expect that this study would be helpful for better understanding of eggshellbased biomineralization.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHOD
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (21)
참고문헌 신청
[학술지(정기간행물)] - Marin, F. / 2008 / Molluscan shell proteins: primary structure, origin, and evolution / Curr. Top. Dev. Biol 80 : 209 ~ 276
[학술지(정기간행물)] - Rose, M. L. H. / 2009 / Protein constituents of the eggshell: Eggshell-specific matrix proteins / Cell. Mol. Life Sci 66 : 2707 ~ 2719
[학술지(정기간행물)] - Miyamoto, H. / 2013 / The diversity of shell matrix proteins:Genome-wide investigation of the pearl oyster / Pinctada fucata. Zool. Sci 30 : 801 ~ 816
[학술지(정기간행물)] - Mann, K. / 2006 / Proteomic analysis of the acid-soluble organic matrix of the chicken calcified eggshell layer / Proteomics 6 : 3801 ~ 3810
[학술지(정기간행물)] - Evans, J. S. / 2012 / Aragonite-associated biomineralization proteins are disordered and contain interactive motifs / Bioinformatics 28 : 3182 ~ 3185
[학술지(정기간행물)] - Rose, M. L. H. / 2009 / Protein constituents of the eggshell: Eggshell-specific matrix proteins / Cell. Mol. Life Sci 66 : 2707 ~ 2719
[학술지(정기간행물)] - Miyamoto, H. / 2013 / The diversity of shell matrix proteins:Genome-wide investigation of the pearl oyster / Pinctada fucata. Zool. Sci 30 : 801 ~ 816
[학술지(정기간행물)] - Mann, K. / 2006 / Proteomic analysis of the acid-soluble organic matrix of the chicken calcified eggshell layer / Proteomics 6 : 3801 ~ 3810
[학술지(정기간행물)] - Evans, J. S. / 2012 / Aragonite-associated biomineralization proteins are disordered and contain interactive motifs / Bioinformatics 28 : 3182 ~ 3185
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UCI(KEPA) : I410-ECN-0101-2016-470-002231731