Cryptosporidium suis Infection in Post-Weaned and Adult Pigs in Shaanxi Province, Northwestern China

Qing Lin; Xing-Ye Wang; Jian-Wen Chen; Ling Ding; Guang-Hui Zhao

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자료유형: 학술저널

저자정보: Qing Lin (Northwest A&F University) Xing-Ye Wang (Northwest A&F University) Jian-Wen Chen (Gansu Agriculture University) Ling Ding (Northwest A&F University) Guang-Hui Zhao (Northwest A&F University)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.53 No.1

발행연도: 2015.2

수록면: 113 - 117 (5page)

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Cryptosporidium spp., ubiquitous enteric parasitic protozoa of vertebrates, recently emerged as an important cause of economic loss and zoonosis. The present study aimed to determine the distribution and species of Cryptosporidium in post-weaned and adult pigs in Shaanxi province, northwestern China. A total of 1,337 fresh fecal samples of postweaned and adult pigs were collected by sterile disposable gloves from 8 areas of Shaanxi province. The samples were examined by Sheather’s sugar flotation technique and microscopy at×400 magnification for Cryptosporidium infection, and the species in positive samples was further identified by PCR amplification of the small subunit (SSU) rRNA gene. A total of 44 fecal samples were successfully amplified by the nested PCR of the partial SSU rRNA, with overall prevalence of 3.3%. The average prevalence of Cryptosporidium infection in each pig farms ranged from 0 to 14.4%. Species identification by sequencing of SSU rRNA gene revealed that 42 (3.1%) samples were Cryptosporidium suis and 2 (0.15%) were Cryptosporidium scrofarum. C. suis had the highest prevalence (7.5%) in growers and the lowest in breeding pigs (0.97%). C. suis was the predominant species in pre-weaned and adult pigs, while C. scrofarum infected pigs older than 3 months only. A season-related difference of C. suis was observed in this study, with the highest prevalence in autumn (5.5%) and the lowest (1.7%) in winter. The present study provided basic information for control of Cryptosporidium infection in pigs and assessment of zoonotic transmission of pigs in Shaanxi province, China.

#Cryptosporidium #pig #SSU rRNA #Shaanxi province #China

참고문헌 (41)

참고문헌 신청

Jex AR / 2008 / Classification of Cryptosporidium species from patients with sporadic cryptosporidiosis by use of sequence-based multilocus analysis following mutation scanning / J Clin Microbiol 46:2252~2262

Tomazic ML / 2013 / Molecular characterization of Cryptosporidium isolates from calves in Argentina / Vet Parasitol 198:382~386

Xiao L / 2010 / Molecular epidemiology of Cryptosporidiosis: an update / Exp Parasitol 124:80~89

Kváč M / 2013 / Cryptosporidium scrofarum n. sp. (Apicomplexa: Cryptosporidiidae) in domestic pigs (Sus scrofa) / Vet Parasitol 191:218~227

Yui T / 2014 / Age-related detection and molecular characterization of Cryptosporidium suis and Cryptosporidium scrofarum in pre- and post-weaned piglets and adult pigs in Japan / Parasitol Res 113:359~365

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