지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.9
- 수록면
- 213 - 223 (11page)
이용수
초록· 키워드
오류제보하기
The objectives of this study are to develop a molecular diagnosis to differentiate serotypes and mating?types of C. neoformans/C. gattii complex isolates from pigeon droppings in Korea and to elucidate molecular epidemiology of the isolates. Phenotypes and genotypes of C. neoformans/C. gattii complex isolates were identified by biochemical properties and PCR using specific CNLAC1 gene, respectively. To classify serotypes and mating?types of C. neoformans/C. gattii complex isolates, the five reference strains and thirty?three isolates in Korea were investigated by restriction fragment length polymorphism (RFLP) analysis using CNLAC1 gene for varieties, by random amplified polymorphic DNA (RAPD) for serotyping, and by PCR using specific primer sets for mating typing. All isolates in Korea were belonged to C. neoformans var. grubii (serotype A) by RFLP and RAPD patterns which showed high sensitivity and specificity. Therefore, RFLP and RFLP were available to differentiate varieties and serotypes of C. neoformans. Amplification patterns of the five reference strains by specific PCR for mating typing were differentiable, and all isolates were classified into MATα. All C. neoformans environmental isolates in Korea were Cr. neoformans serotype A and MATα which is a more virulent pathogen. This study suggests that RFLP and RAPD are rapid and correct molecular diagnosis tools for epidemiology of C. neoformans/C. gattii complex isolates.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (31)
참고문헌 신청
Baró T / 1999 / Serotyping of Cryptococcus neoformans isolates from clinical and environmental sources in Spain / J Clin Microbiol 37:1170~1172
Buchanan KL / 1998 / What makes Cryptococcus neoformans a pathogen? / Emerg Infect Dis 4:71~83
Centers for Disease Control and Prevention (CDC) / 2010 / Emergence of Cryptococcus gattii‐‐Pacific Northwest, 2004‐2010 / MMWR Morb Mortal Wkly Rep 59:865~868
Chae HS / 2012 / Classification of Cryptococcus neoformans and yeast‐like fungus isolates from pigeon droppings by colony phenotyping and ITS genotyping and their seasonal variations in Korea / Avian Dis 56:58~64
Chae HS / 2012 / Rapid direct identification of Cryptococcus neoformans from pigeon droppings by nested polymerase chain reaction using CNLAC1 gene / Poul Sci 91:1983~1989
Buchanan KL / 1998 / What makes Cryptococcus neoformans a pathogen? / Emerg Infect Dis 4:71~83
Centers for Disease Control and Prevention (CDC) / 2010 / Emergence of Cryptococcus gattii‐‐Pacific Northwest, 2004‐2010 / MMWR Morb Mortal Wkly Rep 59:865~868
Chae HS / 2012 / Classification of Cryptococcus neoformans and yeast‐like fungus isolates from pigeon droppings by colony phenotyping and ITS genotyping and their seasonal variations in Korea / Avian Dis 56:58~64
Chae HS / 2012 / Rapid direct identification of Cryptococcus neoformans from pigeon droppings by nested polymerase chain reaction using CNLAC1 gene / Poul Sci 91:1983~1989
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UCI(KEPA) : I410-ECN-0101-2014-500-002673921