지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.9
- 수록면
- 180 - 187 (8page)
이용수
초록· 키워드
오류제보하기
Hypoxia is an integral part of the environment during luteolysis. In this study we examined whether hypoxia could directly stimulate endothelial cells to produce nitric oxide (NO). Endothelial cells were cultured in hypoxic (5% O₂) or normoxic (20% O₂) conditions and the levels of total NO, inducible NO and endothelial NO was measured. We found that hypoxia but not normoxia upregulated NO production. The increased NO levels correlated with increased inducible NO synthase (iNOS) expression whereas expression of endothelial NOS (eNOS) expression remained constant. Addition of the iNOS specific inhibitor 1400W to hypoxic cultures prevented NO production suggesting that hypoxia?induced NO production in endothelial cells was due mainly to upregulation of iNOS. We also found that prostaglandin F<SUB>2α</SUB> (PGF) production was unaffected by hypoxia suggesting that upregulation of NO was not due to increased synthesis of PGF. In summary, we report that endothelial cells cultured under hypoxic conditions produce NO via the iNOS pathway. This study provides the importance of the relation between the hypoxic environment and the induction of NO by endothelial cells during regression of the corpus luteum in the ovary.
목차
INTRODUCTION
MATERIALS AND METHIDS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (35)
참고문헌 신청
Boiti C / 2003 / Regulation of nitric oxide synthase isoforms and role of nitric oxide during prostaglandin F2alpha‐induced luteolysis in rabbits / Reproduction 125:807~816
de Jesus Perez VA / 2012 / Loss of adenomatous poliposis coli‐alpha3 integrin interaction promotes endothelial apoptosis in mice and humans / Circ Res 111:1551~1564
Ford SP / 1981 / Blood flow to the corpus luteum‐bearing ovary and ipsilateral uterine horn of cows during the oestrous cycle and early pregnancy / J Reprod Fertil 62:555~562
Garvey EP / 1997 / 1400W is a slow, tight binding, and highly selective inhibitor of inducible nitric‐oxide synthase in vitro and in vivo / J Biol Chem 272:4959~4963
Graeber TG / 1994 / Hypoxia induces accumulation of p53 protein, but activation of a G1‐phase checkpoint by low‐oxygen conditions is independent of p53 status / Mol Cell Biol 14:6264~6277
de Jesus Perez VA / 2012 / Loss of adenomatous poliposis coli‐alpha3 integrin interaction promotes endothelial apoptosis in mice and humans / Circ Res 111:1551~1564
Ford SP / 1981 / Blood flow to the corpus luteum‐bearing ovary and ipsilateral uterine horn of cows during the oestrous cycle and early pregnancy / J Reprod Fertil 62:555~562
Garvey EP / 1997 / 1400W is a slow, tight binding, and highly selective inhibitor of inducible nitric‐oxide synthase in vitro and in vivo / J Biol Chem 272:4959~4963
Graeber TG / 1994 / Hypoxia induces accumulation of p53 protein, but activation of a G1‐phase checkpoint by low‐oxygen conditions is independent of p53 status / Mol Cell Biol 14:6264~6277
이 논문의 저자 정보
이 논문과 함께 이용한 논문
(E)-1-(2-(2-nitrovinyl)phenyl)pyrrolidine inhibits Inducible Nitric Oxide Synthase Expression in RAW264.7 Macrophages Stimulated with Lipopolysaccharide
Gyo-Jeong Gu
,
Sang-Hoon Eom
,
Chang Won Suh
외 3명
대한의생명과학회지
2013
.06
Gene Profiling in Osteoclast Precursors by RANKL Using Microarray
Na Kyung Lee
대한의생명과학회지
2013
.06
Interleukin-2 Inhibits Secretin-Induced Bile Secretion in Cholangiocytes
Yoo-Seung Ko
,
Seock-Yeon Hwang
,
Jae-Seung Pa
외 1명
대한의생명과학회지
2013
.06
Performance of Quantitative Real-Time PCR for Detection of Tuberculosis in Granulomatous Lymphadenitis Using Formalin-Fixed Paraffin-Embedded Tissue
Jijgee Munkhdelge
,
Khalilullah Mia-Jan
,
Dongsup Lee
외 9명
대한의생명과학회지
2013
.06
Inhibitory Effects of Curcuminoids on 17β-hydroxysteroid Dehydrogenase Type 1 Activity in Animal Livers
Sung-Eun Lee
,
Byeoung-Soo Pa
,
k
외 3명
대한의생명과학회지
2013
.06
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2014-500-002673885