The mealworm(M) and fly(F) could be used in poultry animal diet as a protein supplementation. One-hundred layer chickens (Hyline, 2-day old) were randomly assigned in 5 treatments. The treatments were T? (control), T₁ (1.0% M), T₂ (2.0% M), T₃ (1.0% M + 1.0% F), T₄ (2.0% F) supplement with 2 replicate (10 birds per replicate) for 6 weeks. Feed consumption was measured daily and body weight was measured twice a week. Carcass characteristics were analysed individually. Growth performance and feed efficiency were high in mealworm supplement (T₁, T₂) but were low in fly supplement (T₃, T₄) treatment(p<0.05). These result was similar in carcass characteristics. However, there were no differences in visceral organs. In result, mealworm and fly supplementation were improve growth performance and carcass characteristics without detrimental effects on visceral organs in layer chicken during growing period for 6 weeks.