지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Objective : This study aimed to ascertain the curative effects of Gamisipjeon-tang (GST) used for wound healing on the skin regeneration of deep second degree burns in mice.
Material & Methods : In vitro, the IκB kinase (IKK) mRNA expression, inducible nitric oxide κ synthase (iNOS) mRNA expression, and cyclooxygenase-2 (COX-2) mRNA expression in the GST concentration from 1 mg/㎖ to 10 mg/㎖ were measured. In vivo, the mice were divided into four groups : the normal group, the BE group (burn-elicited group, control group), the DC group (Duoderm CGF-treated group after burn elicitation), and the GST group (Gamisipjeon-tang treated group after burn elicitation). To determine the anti-inflammatory effects, nuclear factor (NF)-κB p65, iNOS, COX-2 positive reaction were measured by immunohistochemistry. To estimate the skin regenerative effects, change of burn area, 5-bromo-2‘-deoxyuridine (BrdU), and fibroblast growth factor (FGF) positive reaction were analyzed.
Results : In vitro, the iNOS, IKK, COX-2 mRNA expression decreased according to the increase of GST concentration. The significant decrease of COX-2, iNOS, NF-κB positive reaction were the highest in the GST group, followed by the DC group and the BE group (p<0.05). The diameter of burn area was significantly decreased in the GST group as compared to that in the DC and BE group (p<0.05). The BrdU and FGF positive reaction increased more significantly in the GST group than in the DC group, and more significantly in the DC group than in the BE group on the 3rd and 7th day after burn (p<0.05). FGF positive reaction increased in the BE and DC group, whereas it decreased significantly in the GST group on the 14th day (p<0.05). The BrdU positive reaction increased in the BE group, whereas it decreased significantly in the DC and GST group on the 14th day (p<0.05).
Conclusions : This study shows that GST could decrease the inflammatory response and accelerate the skin regeneration as compared to the duoderm CGF in mice with deep second degree burns.
Material & Methods : In vitro, the IκB kinase (IKK) mRNA expression, inducible nitric oxide κ synthase (iNOS) mRNA expression, and cyclooxygenase-2 (COX-2) mRNA expression in the GST concentration from 1 mg/㎖ to 10 mg/㎖ were measured. In vivo, the mice were divided into four groups : the normal group, the BE group (burn-elicited group, control group), the DC group (Duoderm CGF-treated group after burn elicitation), and the GST group (Gamisipjeon-tang treated group after burn elicitation). To determine the anti-inflammatory effects, nuclear factor (NF)-κB p65, iNOS, COX-2 positive reaction were measured by immunohistochemistry. To estimate the skin regenerative effects, change of burn area, 5-bromo-2‘-deoxyuridine (BrdU), and fibroblast growth factor (FGF) positive reaction were analyzed.
Results : In vitro, the iNOS, IKK, COX-2 mRNA expression decreased according to the increase of GST concentration. The significant decrease of COX-2, iNOS, NF-κB positive reaction were the highest in the GST group, followed by the DC group and the BE group (p<0.05). The diameter of burn area was significantly decreased in the GST group as compared to that in the DC and BE group (p<0.05). The BrdU and FGF positive reaction increased more significantly in the GST group than in the DC group, and more significantly in the DC group than in the BE group on the 3rd and 7th day after burn (p<0.05). FGF positive reaction increased in the BE and DC group, whereas it decreased significantly in the GST group on the 14th day (p<0.05). The BrdU positive reaction increased in the BE group, whereas it decreased significantly in the DC and GST group on the 14th day (p<0.05).
Conclusions : This study shows that GST could decrease the inflammatory response and accelerate the skin regeneration as compared to the duoderm CGF in mice with deep second degree burns.
목차
Introduction
Experiment
Results
Discussion and Conclusion
References
참고문헌 (59)
참고문헌 신청
1. [학술지(정기간행물)] - Lu K / 2004 / Study on the management of postburn pathological scars / Chin J Burns. 20 (2) : 65 ~ 66
2. [학술지(정기간행물)] - Wu Z / 2004 / Critical aspects in the treatment of postburn scars / Chin J Burns 20 (2) : 67 ~ 68
3. [학술지(정기간행물)] - Kirsner RS / 1993 / The biology of skin grafts: skin graft as pharmacology agents / Arch Dermatol 129 : 481 ~ 483
4. [학술지(정기간행물)] - Balusubramani M / 2001 / Skin substitute: a review / Burns 27 : 534 ~ 544
5. [단행본] - Korean research group for wound care / 2002 / Advances in wound care / Korea medical book : 315 ~
2. [학술지(정기간행물)] - Wu Z / 2004 / Critical aspects in the treatment of postburn scars / Chin J Burns 20 (2) : 67 ~ 68
3. [학술지(정기간행물)] - Kirsner RS / 1993 / The biology of skin grafts: skin graft as pharmacology agents / Arch Dermatol 129 : 481 ~ 483
4. [학술지(정기간행물)] - Balusubramani M / 2001 / Skin substitute: a review / Burns 27 : 534 ~ 544
5. [단행본] - Korean research group for wound care / 2002 / Advances in wound care / Korea medical book : 315 ~
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UCI(KEPA) : I410-ECN-0101-2014-519-000262498