지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Objectives : The present study was performed to evaluate the potential effects of Bupleuri radix (SH) on regenerative activities in the peripheral sciatic nerve after crushing injury in rats.
Methods : Axonal regeneration after crush injury in rats was analyzed by immunofluorescence staining using anti-NF-200 antibody and retrograde tracing of DiI-axons. Changes in protein levels in the sciatic nerve axons and DRG tissue were analyzed by Western blot analysis and immunofluorescence staining. Effects of SH extract treatment on neurite outgrowth was examined by immunofluorescence staining for cultured DRG neurons.
Results : Major findings on the effects of SH extract treatment on axonal regeneration are summarized as follows.
1. SH-mediated enhancement in axonal regeneration was identified by immuno- fluorescence straining of NF-200 protein and retrograde tracing of DiI-labeled axons.
2. Axonal GAP-43 protein levels were upregulated by SH not only in the injured axons but also in the DRG sensory neurons corresponding to sciatic sensory axons.
3. Phospho-Erk1/2 protein levels were increased in both injured axonal area and DRG sensory neurons by SH. Phospho-Erk1/2 was also found in non-neuronal cells in the injured axons.
4. SH elevated levels of Cdc2 protein produced in Schwann cells in the distal portions of injured sciatic nerves.
5. The neurite outgrowth of DRG sensory neurons in culture was augmented by SH, and these changes were positively associated with GAP-43 production levels in the DRG neurons.
Conclusions: These data suggest that SH extract improves the regenerative responses of injured peripheral neurons, and thus may be useful for understanding molecular basis for the development of therapeutic strategies.
Methods : Axonal regeneration after crush injury in rats was analyzed by immunofluorescence staining using anti-NF-200 antibody and retrograde tracing of DiI-axons. Changes in protein levels in the sciatic nerve axons and DRG tissue were analyzed by Western blot analysis and immunofluorescence staining. Effects of SH extract treatment on neurite outgrowth was examined by immunofluorescence staining for cultured DRG neurons.
Results : Major findings on the effects of SH extract treatment on axonal regeneration are summarized as follows.
1. SH-mediated enhancement in axonal regeneration was identified by immuno- fluorescence straining of NF-200 protein and retrograde tracing of DiI-labeled axons.
2. Axonal GAP-43 protein levels were upregulated by SH not only in the injured axons but also in the DRG sensory neurons corresponding to sciatic sensory axons.
3. Phospho-Erk1/2 protein levels were increased in both injured axonal area and DRG sensory neurons by SH. Phospho-Erk1/2 was also found in non-neuronal cells in the injured axons.
4. SH elevated levels of Cdc2 protein produced in Schwann cells in the distal portions of injured sciatic nerves.
5. The neurite outgrowth of DRG sensory neurons in culture was augmented by SH, and these changes were positively associated with GAP-43 production levels in the DRG neurons.
Conclusions: These data suggest that SH extract improves the regenerative responses of injured peripheral neurons, and thus may be useful for understanding molecular basis for the development of therapeutic strategies.
목차
서론
실험
성적
고찰
결론
참고문헌
참고문헌 (63)
참고문헌 신청
1. [학술지(정기간행물)] - Han IS / 2007 / Cdc2-mediated Schwann cell migration during peripheral nerve regeneration / J Cell Sci 15 (120) : 246 ~ 255
2. [학술지(정기간행물)] - Doree M / 1994 / The cyclin-dependent protein kinases and the control of cell division / FASEB J 8 (14) : 1114 ~ 1121
3. [학술지(정기간행물)] - Doree M / 2002 / From Cdc2 to Cdk1: when did the cell cycle kinase join its cyclin partner? / J Cell Sci 15 (115) : 2461 ~ 2464
4. [학술지(정기간행물)] - Pines J / 1999 / Four-dimensional control of the cell cycle / Nat Cell Biol 1 : E73 ~ E79
5. [학술지(정기간행물)] - Roskoski R / 2005 / Src kinase regulation by phosphorylation and dephosphorylation / Biochem Biophys Res Commun 27 (331) : 1 ~ 14
2. [학술지(정기간행물)] - Doree M / 1994 / The cyclin-dependent protein kinases and the control of cell division / FASEB J 8 (14) : 1114 ~ 1121
3. [학술지(정기간행물)] - Doree M / 2002 / From Cdc2 to Cdk1: when did the cell cycle kinase join its cyclin partner? / J Cell Sci 15 (115) : 2461 ~ 2464
4. [학술지(정기간행물)] - Pines J / 1999 / Four-dimensional control of the cell cycle / Nat Cell Biol 1 : E73 ~ E79
5. [학술지(정기간행물)] - Roskoski R / 2005 / Src kinase regulation by phosphorylation and dephosphorylation / Biochem Biophys Res Commun 27 (331) : 1 ~ 14
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UCI(KEPA) : I410-ECN-0101-2014-519-000280970