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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.3
- 수록면
- 41 - 45 (5page)
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Purpose: Acute fulminant hepatic failure (FHF) is associated with high mortality. Recent studies have shown that a liver support system based on viable hepatocytes can prolong life in animal models of FHF and also in some FHF patients who were successfully bridged to liver transplantation. To be applied in humans, the bioartificial liver (BAL) system should have sufficient liver cell mass to provide adequate bioactive support. The most widely investigated bioreactor at present is based on hollow fiber membrane. However, it is difficult to build a scaledup module of this model. We devised a fluidized-bed bioreactor that is packed with isolated porcine hepatocytes, and these hepatcytes are immobilized in Ca-alginate hydrogel.
Methods: We isolated fresh porcine hepatocytes using a 2-step collagenase perfusion method, and they were suspended in 1.5% alginate solution. Through a drop-generator, this mixture was gelled in 135mM Cacl2. The resulting spherical beads (mean size: 500 m) were embedded in a module. An average of 2x1010 hepatocytes were present in the module. The efficacy of our design was tested in pigs that had undergone total hepatic devascularization and portocaval shunt.
Results: The BAL-treated group showed a significantly lower ammonium build-up rate compared to the control group (598.6±344.2 g/dl vs 1937.6±744.1 g/dl, respectively, at 8 hours after connecting to BAL). In addition, the intracranial pressure was well controlled in the BAL-treated group, whereas the control group showed a progressive increase of the intracranial pressure (16.9±1mmH2O vs 21.9±2.6mmH2O, respectively, at 8 hours after connecting to BAL).
Conclusion: Our bioartificial liver system is a fluidized-bed bioreactor packed with immobilized porcine hepatocytes, and it seems to be a more effective scaled-up module.
Methods: We isolated fresh porcine hepatocytes using a 2-step collagenase perfusion method, and they were suspended in 1.5% alginate solution. Through a drop-generator, this mixture was gelled in 135mM Cacl2. The resulting spherical beads (mean size: 500 m) were embedded in a module. An average of 2x1010 hepatocytes were present in the module. The efficacy of our design was tested in pigs that had undergone total hepatic devascularization and portocaval shunt.
Results: The BAL-treated group showed a significantly lower ammonium build-up rate compared to the control group (598.6±344.2 g/dl vs 1937.6±744.1 g/dl, respectively, at 8 hours after connecting to BAL). In addition, the intracranial pressure was well controlled in the BAL-treated group, whereas the control group showed a progressive increase of the intracranial pressure (16.9±1mmH2O vs 21.9±2.6mmH2O, respectively, at 8 hours after connecting to BAL).
Conclusion: Our bioartificial liver system is a fluidized-bed bioreactor packed with immobilized porcine hepatocytes, and it seems to be a more effective scaled-up module.
목차
서론
실험 대상 및 방법
결과
고찰
결론
참고문헌
참고문헌 (14)
참고문헌 신청
1. [학술지(정기간행물)] - Sheil AGR / 1991 / Acute and subacute fulminant hepatic failure; the role of liver transplantation / Med J Aust 154 : 724 ~ 728
2. [학술지(정기간행물)] - Lidofsky S / 1993 / Liver transplantation for fulminant hepatic failure / Gastroenterol Clin North AM 22 : 257 ~ 269
3. [단행본] - Guguen-Guillouzo C / 1986 / Les Editions INSERM / John Libbey Eurotext
4. [학술지(정기간행물)] - Sussman NL / 1994 / The hepatix extracorporeal liver assist device:initial clinical experience / Artif Organs 18 : 390 ~ 396
5. [학술지(정기간행물)] - Demetriou AA / 1995 / Early clinical experience with hybrid bioartificial liver, Scand / J. Gastroenterol 30 : 111 ~ 117
2. [학술지(정기간행물)] - Lidofsky S / 1993 / Liver transplantation for fulminant hepatic failure / Gastroenterol Clin North AM 22 : 257 ~ 269
3. [단행본] - Guguen-Guillouzo C / 1986 / Les Editions INSERM / John Libbey Eurotext
4. [학술지(정기간행물)] - Sussman NL / 1994 / The hepatix extracorporeal liver assist device:initial clinical experience / Artif Organs 18 : 390 ~ 396
5. [학술지(정기간행물)] - Demetriou AA / 1995 / Early clinical experience with hybrid bioartificial liver, Scand / J. Gastroenterol 30 : 111 ~ 117
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UCI(KEPA) : I410-ECN-0101-2014-514-001248240