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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.12
- 수록면
- 205 - 214 (10page)
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Purpose: The most important consideration for therapy using MSCs is the differentiation of the target organ’s cell type. For in-vitro hepatogenic differentiation of MSCs, the main focus is efficient induction of the MSCs into the endoderm stage. Activin A, which is a signaling molecule that is similar to Nodal, promotes the induction of definitive endoderm from both ESs and MSCs. The protocols for induction into definitive endoderm have shown different efficiency and reproducibility depending on the researchers or the sources of the MSCs. Thus, a study on the various conditions of Activin A is needed to efficiently differentiate MSCs into the definitive endoderm lineage of MSCs.
Methods: MSCs were isolated from human adipose tissues and these were cultured in MCM (MSCs Culture Medium) on a human fibronectin coated plate. At 70∼80% confluence, the MSCs were harvested and cultured in MCM supplemented with Activin A, at a 50 ng/mL concentration, and FGF4. The expression of the genes related with MSCs or primitive endoderm were analyzed by RT-PCR. The changes of cell morphology for differentiation were also observed by a light microscope & a SEM.
Results: The expression of genes related with primitive foregut endoderm was seen in the groups that were treated with a higher concentration of Activin A. The morphology of the cells that differentiated into definitive endoderm were not different from those of the undifferentiated MSCs. The expression of genes related with functional primitive hepatocytes was seen in the early phase during hepatic differentiation. The cell morphology was changed to a similar cuboidal form in a time-dependent manner.
Conclusion: Activin A promotes a more rapid induction of definitive endoderm. It also makes an efficient condition for the differentiation into primitive foregut endoderm at a higher concentration.
Methods: MSCs were isolated from human adipose tissues and these were cultured in MCM (MSCs Culture Medium) on a human fibronectin coated plate. At 70∼80% confluence, the MSCs were harvested and cultured in MCM supplemented with Activin A, at a 50 ng/mL concentration, and FGF4. The expression of the genes related with MSCs or primitive endoderm were analyzed by RT-PCR. The changes of cell morphology for differentiation were also observed by a light microscope & a SEM.
Results: The expression of genes related with primitive foregut endoderm was seen in the groups that were treated with a higher concentration of Activin A. The morphology of the cells that differentiated into definitive endoderm were not different from those of the undifferentiated MSCs. The expression of genes related with functional primitive hepatocytes was seen in the early phase during hepatic differentiation. The cell morphology was changed to a similar cuboidal form in a time-dependent manner.
Conclusion: Activin A promotes a more rapid induction of definitive endoderm. It also makes an efficient condition for the differentiation into primitive foregut endoderm at a higher concentration.
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고찰
참고문헌
참고문헌 (30)
참고문헌 신청
1. [학술지(정기간행물)] - Barry FP / 2004 / Mesenchymal stem cells: clinical applications and biological characterization / Int J Biochem Cell Biol 36 : 568 ~ 584
2. [학술지(정기간행물)] - Kume S / 2005 / Stem-cell-based approaches for regenerative medicine / Dev Growth Differ 47 : 393 ~ 402
3. [학술지(정기간행물)] - Denker HW / 2006 / Potentiality of embryonic stem cells: an ethical problem even with alternative stem cell sources / J Med Ethics 32 : 665 ~ 871
4. [학술지(정기간행물)] - Takahashi K / 2006 / Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by\ defined factors / Cell 126 : 663 ~ 676
5. [학술지(정기간행물)] - Miura K / 2009 / Variation in the safety of induced pluripotent stem cell lines / Nat Biotechnol 27 : 743 ~ 745
2. [학술지(정기간행물)] - Kume S / 2005 / Stem-cell-based approaches for regenerative medicine / Dev Growth Differ 47 : 393 ~ 402
3. [학술지(정기간행물)] - Denker HW / 2006 / Potentiality of embryonic stem cells: an ethical problem even with alternative stem cell sources / J Med Ethics 32 : 665 ~ 871
4. [학술지(정기간행물)] - Takahashi K / 2006 / Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by\ defined factors / Cell 126 : 663 ~ 676
5. [학술지(정기간행물)] - Miura K / 2009 / Variation in the safety of induced pluripotent stem cell lines / Nat Biotechnol 27 : 743 ~ 745
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UCI(KEPA) : I410-ECN-0101-2014-514-001253354