지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.6
- 수록면
- 87 - 93 (7page)
이용수
초록· 키워드
오류제보하기
Purpose: To investigate the differentiation of rat bone marrow-derived mesenchymal stem cells (MSCs) into hepatocytes by cell transplantation using fibrin gels in a 70% hepatectomized rat model.
Methods: MSCs were isolated from Sprague-Dawley rats. MSCs (1.5×107 cells) were mixed with fibrin gels and injected immediately into the abdominal cavity of 70% hepatectomized rats. Fibrin-gels consisted of 500 IU/ml of thrombin and 90 mg/ml of fibrinogen. Transplanted MSCs in the fibrin scaffold were retrieved from surgically opened peritoneal cavities of rats on days 5, 10, 15, and 21 after the operation. The specimens were analyzed histologically and immunohistochemically.
Results: On H&E staining, MSCs from hepatectomized rats had changed to a round shape, while MSCs of the control group kept their spindle shape. When the fibrin matrix was biodegraded at day 15, the morphology of the MSCs had changed to hepatocyte-like cells without sinusoids and the hepatocyte-like cells had formed a three-dimensional tissue permitting cell-to-cell contacts within the matrix. On immunohistochemistry, MSCs expressed the hepatocyte markers cytokeratin 18, albumin, and alpha-fetoprotein, after 15 days of transplantation.
Conclusion: When bone marrow-derived MSCs are transplanted using fibrin gels in the 70% hepatectomized rat, MSCs differentiate into hepatocyte-like cells and are conglomerated so that they form three-dimensional tissue-like hepatocytes without sinusoids.
Methods: MSCs were isolated from Sprague-Dawley rats. MSCs (1.5×107 cells) were mixed with fibrin gels and injected immediately into the abdominal cavity of 70% hepatectomized rats. Fibrin-gels consisted of 500 IU/ml of thrombin and 90 mg/ml of fibrinogen. Transplanted MSCs in the fibrin scaffold were retrieved from surgically opened peritoneal cavities of rats on days 5, 10, 15, and 21 after the operation. The specimens were analyzed histologically and immunohistochemically.
Results: On H&E staining, MSCs from hepatectomized rats had changed to a round shape, while MSCs of the control group kept their spindle shape. When the fibrin matrix was biodegraded at day 15, the morphology of the MSCs had changed to hepatocyte-like cells without sinusoids and the hepatocyte-like cells had formed a three-dimensional tissue permitting cell-to-cell contacts within the matrix. On immunohistochemistry, MSCs expressed the hepatocyte markers cytokeratin 18, albumin, and alpha-fetoprotein, after 15 days of transplantation.
Conclusion: When bone marrow-derived MSCs are transplanted using fibrin gels in the 70% hepatectomized rat, MSCs differentiate into hepatocyte-like cells and are conglomerated so that they form three-dimensional tissue-like hepatocytes without sinusoids.
목차
서론
방법
결과
고찰
결론
참고문헌
참고문헌 (22)
참고문헌 신청
1. [학술지(정기간행물)] - Caplan AI / 1991 / Mesenchymal stem cells / J Orthop Res 9 : 641 ~ 650
2. [학술지(정기간행물)] - Ahdjoudj S / 2001 / Reciprocal control of osteoblast/chondroblast and osteoblast/adipocyte differentiation of multipotential clonal human marrow stromal F/STRO-1(＋) cells / J Cell Biochem 81 : 23 ~ 38
3. [학술지(정기간행물)] - Allan EH / 2003 / Differentiation potential of a mouse bone marrow stromal cell line / J Cell Biochem 90 : 158 ~ 169
4. [학술지(정기간행물)] - Wislet-Gendebien S / 2003 / Regulation of neural markers nestin and GFAP expression by cultivated bone marrow stromal cells / J Cell Sci 116 : 3295 ~ 3302
5. [학술지(정기간행물)] - Orlic D / 2003 / Adult bone marrow stem cells regenerate myocardium in ischemic heart disease / Ann N Y Acad Sci 996 : 152 ~ 157
2. [학술지(정기간행물)] - Ahdjoudj S / 2001 / Reciprocal control of osteoblast/chondroblast and osteoblast/adipocyte differentiation of multipotential clonal human marrow stromal F/STRO-1(＋) cells / J Cell Biochem 81 : 23 ~ 38
3. [학술지(정기간행물)] - Allan EH / 2003 / Differentiation potential of a mouse bone marrow stromal cell line / J Cell Biochem 90 : 158 ~ 169
4. [학술지(정기간행물)] - Wislet-Gendebien S / 2003 / Regulation of neural markers nestin and GFAP expression by cultivated bone marrow stromal cells / J Cell Sci 116 : 3295 ~ 3302
5. [학술지(정기간행물)] - Orlic D / 2003 / Adult bone marrow stem cells regenerate myocardium in ischemic heart disease / Ann N Y Acad Sci 996 : 152 ~ 157
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Therapeutic potentials occurring during the early differentiation process of mesenchymal stem cells in a rats model with thioacetamide - induced liver fibrosis
Annals of Hepato-Biliary-Pancreatic Surgery
2013 .03
-
임상적용을 위한 세포치료제로서의 성체 중간엽줄기세포
영남의대학술지
2009 .01
-
Isolation and characterization of deer-derived mesenchymal stem cells
예방수의학회지
2019 .01
-
비과 영역에서 중간엽줄기세포의 적용
대한이비인후-두경부외과학회지
2014 .01
-
Modulation of osteoblastic/odontoblastic differentiation of adult mesenchymal stem cells through gene introduction: a brief review
대한구강악안면외과학회지
2013 .01
-
The Therapeutic Effect of Human Embryonic Stem Cell-Derived Multipotent Mesenchymal Stem Cells on Chemical-Induced Cystitis in Rats
International Neurourology Journal
2018 .01
-
Differentiation Potential of Mesenchymal Stem Cells Is Related to Their Intrinsic Mechanical Properties
International Neurourology Journal
2017 .01
-
Growth Inhibitory Effect of Palatine Tonsil-derived Mesenchymal Stem Cells on Head and Neck Squamous Cell Carcinoma Cells
Clinical and Experimental Otorhinolaryngology
2012 .01
-
호흡기 질환에서의 중간엽 줄기 세포의 치료
대한내과학회지
2015 .01
-
Characteristics of mesenchymal stem cells derived from different tissues of goat
예방수의학회지
2019 .01
-
Bone Marrow-Derived Mesenchymal Stem Cell Therapy as a Candidate Disease-Modifying Strategy in Parkinson’s Disease and Multiple System Atrophy
Journal of Clinical Neurology
2009 .01
-
Neurorestoration Induced by Mesenchymal Stem Cells: Potential Therapeutic Mechanisms for Clinical Trials
Yonsei Medical Journal
2012 .01
-
Regulatory Dendritic Cells Induced by Mesenchymal Stem Cells Ameliorate Dextran Sodium Sulfate-Induced Chronic Colitis in Mice
Gut and Liver
2018 .01
-
Immune Tolerance of Human Dental Pulp-Derived Mesenchymal Stem Cells Mediated by CD4+CD25+ FoxP3+ Regulatory T-Cells and Induced by TGF-β1 and IL-10
Yonsei Medical Journal
2017 .01
-
Differentiation potential of mesenchymal stem cells through electrical stimulation
예방수의학회지
2019 .01
-
개 지방세포 유래의 중간엽 줄기세포의 종양형성시험
Toxicological Research
2007 .09
-
Canine mesenchymal stem cells immunomodulate atopic dermatitis through the induction of regulatory T cells in an ex vivo experimental study
예방수의학회지
2016 .01
이 논문의 저자 정보
이 논문과 함께 이용한 논문
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2014-514-001278057