지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.12
- 수록면
- 481 - 489 (9page)
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초록· 키워드
오류제보하기
Purpose: It is known that the wild-type p53 (w-p53) gene has several functions such as suppression of tumor cell growth, control of the cell cycle, stabilization of the genes and cellular differentiation. Recombinant w-p53 adenovirus was transfected and 5-FU was administered into the LoVo (w-p53 gene positive) and SW-837 (mutant-p53 gene positive) colon cancer cell lines to determine the cell death effects according to the presence or absence of the w-p53 gene.
Methods: The transduction of the p53 gene was done using recombinant adenovirus and liposomes, and the cell death effect was determined by performing MTT assay.
Results: The cell death effect by 5-FU was higher in the LoVo cell line than that in the SW-837 cell line. The rate of w-p53 gene transduction was about 90%. The cell death effect by w-p53 gene transduction was shown by the administration of 10μM of 5-FU. The cell death effect according to the administration of 5-FU after w-p53 gene transduction was 10 fold of that with 5-FU administration in the both cell lines, and there was a more significant effect in the LoVo cell line.
Conclusion: The cell killing effect by 5-FU administration after w-p53 gene transduction showed a synergistically higher effect than those of w-p53 transduction only or 5-FU administration only in the colon cancer cell lines. Gene therapy using w-p53 gene transduction requires more extensive clinical trials.
Methods: The transduction of the p53 gene was done using recombinant adenovirus and liposomes, and the cell death effect was determined by performing MTT assay.
Results: The cell death effect by 5-FU was higher in the LoVo cell line than that in the SW-837 cell line. The rate of w-p53 gene transduction was about 90%. The cell death effect by w-p53 gene transduction was shown by the administration of 10μM of 5-FU. The cell death effect according to the administration of 5-FU after w-p53 gene transduction was 10 fold of that with 5-FU administration in the both cell lines, and there was a more significant effect in the LoVo cell line.
Conclusion: The cell killing effect by 5-FU administration after w-p53 gene transduction showed a synergistically higher effect than those of w-p53 transduction only or 5-FU administration only in the colon cancer cell lines. Gene therapy using w-p53 gene transduction requires more extensive clinical trials.
목차
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (20)
참고문헌 신청
1. [학술지(정기간행물)] - Doci R / 1991 / One hundred patients with hepatic metastases from colorectal cancer treated by resection: analysis of prognostic determinants / Br J Surg 78 : 797 ~ 801
2. [학술지(정기간행물)] - Pawlik TM / 2003 / Combined resection and radiofrequency ablation for advanced hepatic malignancies: results in 172 patients / Ann Surg Oncol 10 : 1059 ~ 1069
3. [단행본] - Wren SM. / 2002 / Non-operative treatment of hepatic metastasis from colorectal carcinoma, In Surgical Oncology: Multidisciplinary Approach to Difficult Problems. 1st ed / Arnold publication : 668 ~ 681
4. [학술지(정기간행물)] - Weber SM / 2000 / Survival after resection of multiple hepatic colorectal metastases / Ann Surg Oncol 7 : 643 ~ 650
5. [학술지(정기간행물)] - Aoki K / 1995 / Liposomemediated in vivo gene transfer of antisense K-ras construct inhibits pancreatic tumor dissemination in the murine peritoneal cavity / Cancer Res 55 : 3810 ~ 3816
2. [학술지(정기간행물)] - Pawlik TM / 2003 / Combined resection and radiofrequency ablation for advanced hepatic malignancies: results in 172 patients / Ann Surg Oncol 10 : 1059 ~ 1069
3. [단행본] - Wren SM. / 2002 / Non-operative treatment of hepatic metastasis from colorectal carcinoma, In Surgical Oncology: Multidisciplinary Approach to Difficult Problems. 1st ed / Arnold publication : 668 ~ 681
4. [학술지(정기간행물)] - Weber SM / 2000 / Survival after resection of multiple hepatic colorectal metastases / Ann Surg Oncol 7 : 643 ~ 650
5. [학술지(정기간행물)] - Aoki K / 1995 / Liposomemediated in vivo gene transfer of antisense K-ras construct inhibits pancreatic tumor dissemination in the murine peritoneal cavity / Cancer Res 55 : 3810 ~ 3816
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UCI(KEPA) : I410-ECN-0101-2013-514-002682904