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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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RNase E plays a major role in the degradation and processing of a large number of RNA transcripts in Escherichia coli and forms the core component of the degradosome, a large protein complex involved in RNA metabolism. RraA and RraB are recently discovered protein inhibitors of RNase E and are evolutionarily conserved. In this study, we observed that, unlike RraA, overexpression of RraB did not rescue growth arrest of E. coli cells overexpressing RNase E. To examine whether this phenomenon stems from differential inhibitory effects of RraA and RraB on RNase E substrates, we analyzed three in vivo RNase E substrates. The results showed that RraA inhibited RNase E activity more efficiently than RraB on the degradation of RNA I, which controls the copy number of ColE1- type plasmid, and rpsO mRNA encoding ribosomal protein S15, while RraB was unable to inhibit the processing of pM1 RNA, a precursor of the RNA component of RNase P, by RNase E. Our results imply that RraB inhibits RNase E activity in a more substrate-dependent manner than RraA and this property of RraB may explain why overexpression of RraB could not rescue cells overexpressing RNase E from growth arrest.
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참고문헌
참고문헌 (18)
참고문헌 신청
1. [학술지(정기간행물)] - Carpousis, A.J. / 2007 / The RNA degradosome of Escherichia coli: an mRNA-degrading machine assembled on RNase E / Annu. Rev. Microbiol 61 : 71 ~ 87
2. [학술지(정기간행물)] - Claverie-martin, F. / 1991 / Analysis of the altered mRNA stability (ams) gene from Escherichia coli / J. Biol. Chem 266 : 2843 ~ 2851
3. [학술지(정기간행물)] - Gao, J. / 2006 / Differential modulation of E. coli mRNA abundance by inhibitory proteins that alter the composition of the degradosome / Mol. Microbiol 61 : 394 ~ 406
4. [학술지(정기간행물)] - Hajnsdorf, E. / 1996 / Multiple degradation pathways of the rpsO mRNA of Escherichia coli RNase E interacts with the 5′ and 3′ extremities of the primary transcript / Biochimie 78 : 416 ~ 424
5. [학술지(정기간행물)] - Jain, C. / 1995 / Autoregulation of RNase E synthesis in Escherichia coli / Nucleic Acids Symp. Ser 33 : 85 ~ 88
2. [학술지(정기간행물)] - Claverie-martin, F. / 1991 / Analysis of the altered mRNA stability (ams) gene from Escherichia coli / J. Biol. Chem 266 : 2843 ~ 2851
3. [학술지(정기간행물)] - Gao, J. / 2006 / Differential modulation of E. coli mRNA abundance by inhibitory proteins that alter the composition of the degradosome / Mol. Microbiol 61 : 394 ~ 406
4. [학술지(정기간행물)] - Hajnsdorf, E. / 1996 / Multiple degradation pathways of the rpsO mRNA of Escherichia coli RNase E interacts with the 5′ and 3′ extremities of the primary transcript / Biochimie 78 : 416 ~ 424
5. [학술지(정기간행물)] - Jain, C. / 1995 / Autoregulation of RNase E synthesis in Escherichia coli / Nucleic Acids Symp. Ser 33 : 85 ~ 88
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UCI(KEPA) : I410-ECN-0101-2013-475-001685216