지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 78 - 85 (8page)
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초록· 키워드
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Endothelial progenitor cells (EPC) are a population of cells that circulate in the blood stream. They play a role in angiogenesis and, therefore, can be prognostic markers of vascular repair. Ginsenoside Rg3 prevents endothelial cell apoptosis through the inhibition of the mitochondrial caspase pathway. It also affects estrogen activity, which reduces EPC senescence. Sun ginseng (SG), which is heat-processed ginseng, has a high content of ginsenosides. The purpose of this study was to investigate the protective effects of SG on senescence-associated apoptosis in EPCs. In order to isolate EPCs, mononuclear cells of human blood buffy coats were cultured and characterized by their uptake of acetylated low-density lipoprotein (acLDL) and their binding of Ulex europaeus agglutinin I (ulex-lectin). Flow cytometry with annexin-V staining was performed in order to assess early and late apoptosis. Senescence was determined by β-galactosidase (β-gal) staining. Staining with 4’-6-Diamidino-2-phenylindole verifi ed that most adherent cells (93±2.7%) were acLDL-positive and ulex-lectin-positive. The percentage of β-gal-positive EPCs was decreased from 93.8±2.0% to 62.5±3.6% by SG treatment. A fl uorescence-activated cell sorter (FACS) analysis showed that 4.9% of EPCs were late apoptotic in controls. Sun ginseng decreased the apoptotic cell population by 39% in the late stage of apoptosis from control baseline levels. In conclusion, these results show antisenescent and antiapoptotic effects of SG in human-derived EPCs, indicating that SG can enhance EPC-mediated repair mechanisms.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES
참고문헌 (54)
참고문헌 신청
Iwaguro, H. / 2002 / Endothelial progenitor cell vascular endothelial growth factor gene transfer for vascular regeneration / Circulation 105:732~738
Assmus, B. / 2002 / Transplantation of Progenitor Cells and Regeneration Enhancement in Acute Myocardial Infarction(TOPCARE-AMI) / Circulation 106:3009~3017
Hill, J. M. / 2003 / Circulating endothelial progenitor cells, vascular function, and cardiovascular risk / N Engl J Med 348:593~600
Circ Res / 2001 / Number and migratory activity of circulating endothelial progenitor cells inversely correlate with risk factors for coronary artery disease / Circ Res 89:E1~E7
Schatteman, G. C. / 2000 / Blood-derived angioblasts accelerate blood-fl ow restoration in diabetic mice / J Clin Invest 106:571~578
Assmus, B. / 2002 / Transplantation of Progenitor Cells and Regeneration Enhancement in Acute Myocardial Infarction(TOPCARE-AMI) / Circulation 106:3009~3017
Hill, J. M. / 2003 / Circulating endothelial progenitor cells, vascular function, and cardiovascular risk / N Engl J Med 348:593~600
Circ Res / 2001 / Number and migratory activity of circulating endothelial progenitor cells inversely correlate with risk factors for coronary artery disease / Circ Res 89:E1~E7
Schatteman, G. C. / 2000 / Blood-derived angioblasts accelerate blood-fl ow restoration in diabetic mice / J Clin Invest 106:571~578
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UCI(KEPA) : I410-ECN-0101-2013-524-001261178