지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.12
- 수록면
- 295 - 306 (12page)
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초록· 키워드
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Objectives : This study was undertaken to examine the metabolomic changes due to gender and diurnal variation at sampling time and to identify an appropriate time point for urine sampling in epidemiologic studies using metabolomic profiles.
Methods : Urine samples were collected twice a day (morning and afternoon) from 20 healthy Korean adults after fasting for 8 hours. The metabolomic assay was investigated using H NMR spectroscopy coupled with the principal components analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The metabolites responsible for differentiation between groups were identified through the loading plot of PLS-DA and quantified using Chenomx NMR Suite with a 600 ㎒ library.
Results : Metabolites responsible for differentiation in gender and sampling time were creatinine, trimethyl anine oxide (TMAO), hippurate, mannitol, citrate and acetoacetate. Dimethylamine showed difference only as a factor of diurnal time. The level of creatinine was higher in men compared to women, and the levels of citrate, TMAO, hippurate, mannitol, and acetoacetate were higher in women compared to men. The levels of creatinine, TMAO, hippurate, dimethylamine and mannitol were higher in the morning rather than the afternoon while those of citrate and acetoacetate were higher in the afternoon rather than the morning.
Conclusions : Since urinary metabolomic profiles varied by gender and diurnal cycle, urine sampling should be performed at the same time point for all participants in epidemiologic studies using metabolomic profiles.
Methods : Urine samples were collected twice a day (morning and afternoon) from 20 healthy Korean adults after fasting for 8 hours. The metabolomic assay was investigated using H NMR spectroscopy coupled with the principal components analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The metabolites responsible for differentiation between groups were identified through the loading plot of PLS-DA and quantified using Chenomx NMR Suite with a 600 ㎒ library.
Results : Metabolites responsible for differentiation in gender and sampling time were creatinine, trimethyl anine oxide (TMAO), hippurate, mannitol, citrate and acetoacetate. Dimethylamine showed difference only as a factor of diurnal time. The level of creatinine was higher in men compared to women, and the levels of citrate, TMAO, hippurate, mannitol, and acetoacetate were higher in women compared to men. The levels of creatinine, TMAO, hippurate, dimethylamine and mannitol were higher in the morning rather than the afternoon while those of citrate and acetoacetate were higher in the afternoon rather than the morning.
Conclusions : Since urinary metabolomic profiles varied by gender and diurnal cycle, urine sampling should be performed at the same time point for all participants in epidemiologic studies using metabolomic profiles.
목차
ABSTRACT
서론
연구방법
연구결과
고찰
감사의 글
참고문헌
참고문헌 (39)
참고문헌 신청
1. [학술지(정기간행물)] - Lenz EM / 2003 / A 1H NMR-based metabonomic study of urine and plasma samples obtained from healthy human subjects / J Pharm Biomed Anal 33 (5) : 1103 ~ 1115
2. [학술지(정기간행물)] - Keun HC / 2006 / Metabonomic modeling of drug toxicity / Pharmacol Ther 109 (1) : 92 ~ 106
3. [학술지(정기간행물)] - Lindon JC / 2003 / Contemporary issues in toxicology the role of metabonomics in toxicology and its evaluation by the COMET project / Toxicol Appl Pharmacol 187 (3) : 137 ~ 146
4. [학술지(정기간행물)] - Laffel L / 1999 / Ketone bodies: a Review of physiology, pathophysiology and application of monitoring to diabetes / Diabetes Metab Res Rev 15 (6) : 412 ~ 426
5. [학술지(정기간행물)] - Kochhar S / 2006 / Probing gender-specific metabolism differences in humans by nuclear magnetic resonance-based metabonomics / Anal Biochem 352 (2) : 274 ~ 281
2. [학술지(정기간행물)] - Keun HC / 2006 / Metabonomic modeling of drug toxicity / Pharmacol Ther 109 (1) : 92 ~ 106
3. [학술지(정기간행물)] - Lindon JC / 2003 / Contemporary issues in toxicology the role of metabonomics in toxicology and its evaluation by the COMET project / Toxicol Appl Pharmacol 187 (3) : 137 ~ 146
4. [학술지(정기간행물)] - Laffel L / 1999 / Ketone bodies: a Review of physiology, pathophysiology and application of monitoring to diabetes / Diabetes Metab Res Rev 15 (6) : 412 ~ 426
5. [학술지(정기간행물)] - Kochhar S / 2006 / Probing gender-specific metabolism differences in humans by nuclear magnetic resonance-based metabonomics / Anal Biochem 352 (2) : 274 ~ 281
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UCI(KEPA) : I410-ECN-0101-2012-539-003787058